Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913566,2770CT38.5% 48.5 / 52.9 52pseudogene (100/279 nt)exoDpseudogene, DLP12 prophage, phage‑type exonuclease family,Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (13/19);  new base T (14/6);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 4.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAG  >  NC_000913/566142‑566415
                                                                                                                                       |                                                                                                                                          
ccTCGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATtttt                                                                                                                                         <  2:240214/139‑1 (MQ=255)
   cGTCCATTTTTTCGATGAACTCCGGCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTcg                                                                                                                                      >  2:684041/1‑139 (MQ=255)
           ttttCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCAATCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCACGCGCGGGTCAAAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                              <  2:581729/139‑1 (MQ=255)
              tCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCcaca                                                                                                                           <  2:844377/139‑1 (MQ=255)
                gATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATg                                                                                                                         <  2:682906/139‑1 (MQ=255)
                      cTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTGACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc                                                                                                                   <  1:533260/139‑1 (MQ=255)
                      cTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc                                                                                                                   <  2:708878/139‑1 (MQ=255)
                          ggCACGATCTCGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTGCCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCa                                                                                                               <  2:482837/139‑1 (MQ=255)
                                   tcGTCAAAACTCGCCATGTACTTTTCATCCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCa                                                                                                      <  1:856322/139‑1 (MQ=255)
                                      tCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc                                                                                                                   >  2:633849/1‑123 (MQ=255)
                                      tCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTAc                                                                                                                   <  1:633849/123‑1 (MQ=255)
                                                          ttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGt                                                                                                                                   >  1:54609/1‑87 (MQ=255)
                                                          ttCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGt                                                                                                                                   <  2:54609/87‑1 (MQ=255)
                                                               tCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAacc                                                                          >  2:186544/1‑139 (MQ=255)
                                                                  gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAaccacc                                                                       >  1:321054/1‑139 (MQ=255)
                                                                  gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAaccacc                                                                       >  1:251363/1‑139 (MQ=255)
                                                                  gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCcaca                                                                                                                           >  1:470275/1‑87 (MQ=255)
                                                                  gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCcaca                                                                                                                           <  2:470275/87‑1 (MQ=255)
                                                                  gCTCAATCACGACATAATGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAaccacc                                                                       >  2:479059/1‑139 (MQ=255)
                                                                   cTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCAcc                                                                                                             >  2:491324/1‑100 (MQ=255)
                                                                   cTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCAcc                                                                                                             <  1:491324/100‑1 (MQ=255)
                                                                      aaTCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGc                                                                   <  2:321054/139‑1 (MQ=255)
                                                                                  aTGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                              <  2:585422/68‑1 (MQ=255)
                                                                                  aTGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCAccc                                                                                                                              >  1:585422/1‑68 (MQ=255)
                                                                                  aTGCAGGCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATg                                                       >  2:201009/1‑139 (MQ=255)
                                                                                        gCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATcc                                                 >  1:127163/1‑139 (MQ=255)
                                                                                        gCCTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATcc                                                 >  1:869027/1‑139 (MQ=255)
                                                                                         ccTTCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATccc                                                >  2:73785/1‑139 (MQ=255)
                                                                                          cTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCCCGAAACCACCGAGCCGGAATTTCATGAAATCCCg                                               <  1:186544/139‑1 (MQ=255)
                                                                                           ttCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCgg                                              <  2:251363/139‑1 (MQ=255)
                                                                                           ttCACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCgg                                              >  2:477233/1‑139 (MQ=255)
                                                                                            tCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCggg                                             <  2:888023/139‑1 (MQ=255)
                                                                                             cACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGc                                                                   <  2:135766/116‑1 (MQ=255)
                                                                                             cACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGc                                                                   >  1:135766/1‑116 (MQ=255)
                                                                                             cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                         <  2:609841/62‑1 (MQ=255)
                                                                                             cACGCTTCATACGCGGGTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                         >  1:609841/1‑62 (MQ=255)
                                                                                                  ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTcgcg                                                                                                                                    >  2:664396/1‑46 (MQ=255)
                                                                                                  ttCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTcgcg                                                                                                                                    <  1:664396/46‑1 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                         >  2:865777/1‑47 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATg                                                                                                                         <  1:865777/47‑1 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGAGGTaaacgggcattt                             >  2:123252/1‑128 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGAGGTaaacgggcattt                             >  1:74319/1‑128 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGAGGTaaacggg                                  <  2:908708/134‑7 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGAGGTaaacggg                                  >  1:908708/1‑128 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGc                                                                                                                        <  2:134806/48‑1 (MQ=255)
                                                                                                            ggTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGc                                                                                                                        >  1:134806/1‑48 (MQ=255)
                                                                                                              tCATAGTTGGCAAAGTACCAGGCATTTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCTGACTTTATGGCCTCGAAACCACCGAGCCGGAACTTCATGAAATCCCGGGAGGTaaacgggcatttca                           >  1:105576/1‑126 (MQ=255)
                                                                                                                       gCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                     <  2:619029/72‑1 (MQ=255)
                                                                                                                       gCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTAtt                                                                                     >  1:619029/1‑72 (MQ=255)
                                                                                                                       gCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGc                  >  2:292358/1‑139 (MQ=255)
                                                                                                                                cAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCg         <  2:894512/139‑1 (MQ=255)
                                                                                                                                  ggCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCg                            <  2:666545/118‑1 (MQ=255)
                                                                                                                                  ggCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCg                            >  1:666545/1‑118 (MQ=255)
                                                                                                                                       cTTTTCGCGTCACCCACATGCTGTACTGCACCTggg                                                                                                         <  2:101919/36‑1 (MQ=255)
                                                                                                                                       cTTTTCGCGTCACCCACATGCTGTACTGCACCTggg                                                                                                         >  1:101919/1‑36 (MQ=255)
                                                                                                                                       cTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATcc          >  2:388996/1‑131 (MQ=255)
                                                                                                                                       cTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATcc          <  1:388996/131‑1 (MQ=255)
                                                                                                                                       cTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCag  <  1:292358/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                          
CCTCGTCCATTTTTTCGATGAACTCCGGCACCATCTCGTCAAAACTCGCCATGTACTTTTCATTCCGCTCAATCACGACATAATGCAGGCCTTCACGCTTCATGCGCGGGTCATAGTTGGCAAAGTACCAGGCATCTTTTCGCGTCACCCACATGCTGTACTGCACCTGGGCCATGTAAGCCGATTTTATTGCCTCGAAACCACCGAGCCGGAATTTCATGAAATCCCGGGAGGTACGAGTATTGCCGGAAGCGTGGCCTGTATCCGGATGCAG  >  NC_000913/566142‑566415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑