Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,826	C→T	A267T (GCA→ACA)	purU ←	formyltetrahydrofolate hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,826	0	C	T	100.0%	148.6 / NA	44	A267T (GCA→ACA)	purU	formyltetrahydrofolate hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/20);  total (24/20)

GCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGT  >  NC_000913/1287690‑1287960                                                                                                                                         |                                                                                                                                       gccgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                      <  2:120672/139‑1 (MQ=255)   cgcTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  2:659075/139‑1 (MQ=255)             tgtTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGtata                                                                                                                          <  2:274961/139‑1 (MQ=255)               ttACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  1:283081/127‑1 (MQ=255)               ttACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    >  2:283081/1‑127 (MQ=255)                  cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                      >  2:143009/1‑122 (MQ=255)                  cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa                                                                                                                     >  1:608624/1‑139 (MQ=255)                  cGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATAGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                      >  1:610863/1‑122 (MQ=255)                                 aCTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGttt                                                                                                      >  2:401117/1‑139 (MQ=255)                                                         ccaggATTAAAGGTAAACAGTTATTTTTTCCGGGGATTAAAGGATAATCGTTCGATTAACGTAAACAAAGACCCCCTGTGTCCGTTATTTGTATAGTGCACGGCTTAAGACGTTTTTCTCGACGTCACGACCTgcgcgc                                                                              <  2:169615/136‑1 (MQ=255)                                                           cGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcat                                                                            >  1:13905/1‑139 (MQ=255)                                                           cGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGcat                                                                            >  2:871778/1‑139 (MQ=255)                                                                tAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                      >  2:106077/1‑76 (MQ=255)                                                                        aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa                                                                                                                     >  1:295402/1‑85 (MQ=255)                                                                        aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCa                                                                                                                     <  2:295402/85‑1 (MQ=255)                                                                        aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACTTTTTTCTGAACTTCAGGACTTGCGCGCATCATACCTTcctat                                                               >  1:169615/1‑135 (MQ=255)                                                                        aCCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCCTATCTTCAg                                                                 >  1:236522/1‑137 (MQ=255)                                                                        aCCAGTTATTTTTGCTGGCAATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:236522/137‑1 (MQ=255)                                                                                    tGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAg                                                                                                            >  1:719801/1‑82 (MQ=255)                                                                                    tGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAg                                                                                                            <  2:719801/82‑1 (MQ=255)                                                                                       tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCa                                                                                                                                      >  2:412683/1‑53 (MQ=255)                                                                                       tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    >  2:348390/1‑55 (MQ=255)                                                                                       tGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  1:348390/55‑1 (MQ=255)                                                                                        ggCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa                                                   <  1:170219/135‑1 (MQ=255)                                                                                        ggCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa                                                   >  2:170219/1‑135 (MQ=255)                                                                                                      aatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa                                                   <  1:886509/121‑1 (MQ=255)                                                                                                      aatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGa                                                   >  2:886509/1‑121 (MQ=255)                                                                                                      aatCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTcc                                 <  1:871778/139‑1 (MQ=255)                                                                                                        tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    >  2:216824/1‑38 (MQ=255)                                                                                                        tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGt                                                                                                                                    <  1:216824/38‑1 (MQ=255)                                                                                                        tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGt                                                                                            <  1:407400/78‑1 (MQ=255)                                                                                                        tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGt                                                                                            >  2:407400/1‑78 (MQ=255)                                                                                                        tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGa                                               >  1:561629/1‑123 (MQ=255)                                                                                                        tCGTTCGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGa                                               <  2:561629/123‑1 (MQ=255)                                                                                                             cGATTACCGTAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCatga                          >  2:800136/1‑139 (MQ=255)                                                                                                                      tAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:317366/91‑1 (MQ=255)                                                                                                                      tAAACAAAGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 >  1:317366/1‑91 (MQ=255)                                                                                                                             aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 >  1:907960/1‑84 (MQ=255)                                                                                                                             aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAg                                                                 <  2:907960/84‑1 (MQ=255)                                                                                                                             aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgtg                                                            <  2:614550/89‑1 (MQ=255)                                                                                                                             aGACGCGCTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCtgtg                                                            >  1:614550/1‑89 (MQ=255)                                                                                                                                 gcgcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGa      <  1:401117/139‑1 (MQ=255)                                                                                                                                  cgcTGTGTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGAt     >  1:750291/1‑139 (MQ=255)                                                                                                                                     tgtgTCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGt  <  2:750291/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GCCGCTGTAAAGTGTTACGTTGAGAAAAATGAACTGGTTGCGTAATTTTCATCCGTAACGGATTAAAGGTAACCAGTTATTTTTGCTGGCGATTAAAGAATAATCGTTCGATTACCGTAAACAAAGACGCGCTGTGCCAGTACTTTGTATAGTGCACGACTTAAGACGTTTTTCTCGACGTCACGACCTGCGCGCATCATATCTTCAGCTGTGTAGGTATGATCGACATGAATAACGTCCTGCATGATGATTGGGCCTTCGTCCAGATTGT  >  NC_000913/1287690‑1287960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑