Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,481,041	A→G	T45A (ACG→GCG)	ynbD →	putative phosphatase inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,481,041	0	A	G	100.0%	136.7 / NA	36	T45A (ACG→GCG)	ynbD	putative phosphatase inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/18);  total (18/18)

TATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTA  >  NC_000913/1480907‑1481175                                                                                                                                       |                                                                                                                                       tATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGc                                                                                                                                    <  1:441575/139‑1 (MQ=255)  aTTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAgcggcg                                                                                                                                   <  1:136031/139‑1 (MQ=255)         cAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCtt                                                                                                                            >  2:430296/1‑139 (MQ=255)           aGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCtttt                                                                                                                          >  1:894757/1‑139 (MQ=255)                ctggctggTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTcc                                                                                                                     <  1:191213/139‑1 (MQ=255)                   gctggTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTg                                                                                                                  <  1:430296/139‑1 (MQ=255)                   gctggTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTg                                                                                                                  >  1:153927/1‑139 (MQ=255)                           ttgttgCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTg                                                                                                          <  2:894757/139‑1 (MQ=255)                             gttgCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  2:153927/139‑1 (MQ=255)                                 cTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCtt                                                                                                    >  1:406272/1‑139 (MQ=255)                                          tttttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGa                                                                                                                                  >  1:427320/1‑100 (MQ=255)                                          tttttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGa                                                                                                                                  <  2:427320/100‑1 (MQ=255)                                            tttCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTg                                                                                         <  2:406272/139‑1 (MQ=255)                                                                           ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGttt                                                          >  2:329661/1‑139 (MQ=255)                                                                           ttCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTAGCTGTTCtttt                                                          >  2:592885/1‑139 (MQ=255)                                                                             cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTgg                                                                                        >  1:406555/1‑107 (MQ=255)                                                                             cACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTgg                                                                                        <  2:406555/107‑1 (MQ=255)                                                                                  cgGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCAc                                                   <  1:592885/139‑1 (MQ=255)                                                                                     ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        >  2:260457/1‑83 (MQ=255)                                                                                     ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  1:260457/83‑1 (MQ=255)                                                                                     ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACt                                                                                                 >  1:743038/1‑90 (MQ=255)                                                                                     ttCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACt                                                                                                 <  2:743038/90‑1 (MQ=255)                                                                                               tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        >  1:875211/1‑73 (MQ=255)                                                                                               tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGtt                                                                                                        <  2:875211/73‑1 (MQ=255)                                                                                               tAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAg                                      >  2:436636/1‑139 (MQ=255)                                                                                                          atatCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGt                           >  2:901087/1‑139 (MQ=255)                                                                                                             tCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAgcg                                    >  1:720183/1‑127 (MQ=255)                                                                                                             tCCCCAGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAgcg                                    <  2:720183/127‑1 (MQ=255)                                                                                                                  aGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATc                                                                                     <  2:135196/73‑1 (MQ=255)                                                                                                                  aGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATc                                                                                     >  1:135196/1‑73 (MQ=255)                                                                                                                  aGTCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGTTTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGc                   >  1:492333/1‑139 (MQ=255)                                                                                                                    tCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTcc                                                                                                      <  2:50672/54‑1 (MQ=255)                                                                                                                    tCAGGTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTcc                                                                                                      >  1:50672/1‑54 (MQ=255)                                                                                                                       ggTATTCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTAtt              <  1:901087/139‑1 (MQ=255)                                                                                                                            tCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                              >  2:496026/1‑118 (MQ=255)                                                                                                                            tCGGTTGGGAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACt                              <  1:496026/118‑1 (MQ=255)                                                                                                                                   ggAAGCGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTa  <  1:436636/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TATTGCTACAAGGCGCTGGCTGGTTATTGTTGCTGGCCCCGTTTTTCTTCTTCACCTATGGATCTCTTAATCAGTTCACCGCGGTTCAGGACCTTAACAGCCATGATATCCCCAGTCAGGTATTCGGTTGGGAAACGGCGATCCCTTTTCTTCCCTGGACTATTGTTCCTTACTGGAGTCTGGATCTTTTATATGGATTTTCGCTGTTCGTTTGTAGCACGACATTCGAACAGCGCCGACTTGTCCACCGGCTTATTCTGGCAACGGTA  >  NC_000913/1480907‑1481175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑