Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,571,850	T→C	T66A (ACC→GCC)	gadB ←	glutamate decarboxylase B, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,571,850	0	T	C	100.0%	53.7 / NA	15	T66A (ACC→GCC)	gadB	glutamate decarboxylase B, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/8);  total (7/8)

AGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTG  >  NC_000913/1571726‑1571966                                                                                                                             |                                                                                                                     aGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTgg                                                                                                        <  1:731982/139‑1 (MQ=255)   aTCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc                                                                                                      <  1:411543/139‑1 (MQ=255)   aTCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc                                                                                                      <  2:349218/139‑1 (MQ=255)                         cgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt                                                                                <  1:802436/139‑1 (MQ=255)                                                             ttGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGa                                                                                          <  1:806707/93‑1 (MQ=255)                                                             ttGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGa                                                                                          >  2:806707/1‑93 (MQ=255)                                                              tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc                                                                               >  1:89758/1‑103 (MQ=255)                                                              tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATc                                                                               <  2:89758/103‑1 (MQ=255)                                                              tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGACCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGAc                                           >  2:562518/1‑139 (MQ=255)                                                                                   tGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGc                                                                                                                 >  1:451885/1‑48 (MQ=255)                                                                                   tGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGc                                                                                                                 <  2:451885/48‑1 (MQ=255)                                                                                   tGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAg                      >  2:797285/1‑139 (MQ=255)                                                                                               tCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc                                                                                                      >  1:400157/1‑47 (MQ=255)                                                                                               tCAATTTGTGGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGcc                                                                                                      <  2:400157/47‑1 (MQ=255)                                                                                                       tgGACATTTTCGTCGTCCCAGGCCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTg  >  2:549440/1‑139 (MQ=255)                                                                                                                             |                                                                                                                     AGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTG  >  NC_000913/1571726‑1571966

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑