Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,629,913	G→A	P334L (CCG→CTG)	ydfI ←	putative NAD‑dependent D‑mannonate oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,629,913	0	G	A	100.0%	133.2 / NA	39	P334L (CCG→CTG)	ydfI	putative NAD‑dependent D‑mannonate oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

GCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGA  >  NC_000913/1629785‑1630029                                                                                                                                 |                                                                                                                     gCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAgcgc                                                                                                                  <  2:754460/133‑1 (MQ=255) gCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAgcgc                                                                                                                  >  1:754460/1‑133 (MQ=255)  cTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTcc                                                                                                           >  2:716267/1‑139 (MQ=255)   tgtgGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCt                                                                                                          >  1:713662/1‑139 (MQ=255)           tttCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATc                                                                                                  <  1:557055/139‑1 (MQ=255)           tttCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATc                                                                                                  <  2:713662/139‑1 (MQ=255)               tGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGc                                                                                              >  2:850247/1‑139 (MQ=255)               tGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGc                                                                                              >  2:238831/1‑139 (MQ=255)                   tACCATCCATCGCAATCTGCCAGGTTCGATGACGTGACTCCGGCTTGCCATAGCGTGCAACTAATCGGTTAGAGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATa                                                                                          <  1:787549/139‑1 (MQ=255)                          catcGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCa                                                                                   <  1:898578/139‑1 (MQ=255)                           atcGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCAt                                                                                  <  1:850247/139‑1 (MQ=255)                                  tCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATa                                                                           <  1:716267/139‑1 (MQ=255)                                              gATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAATGTCAGCGCTTGTTCCTGCAACATc                                                                                                  <  1:619882/104‑1 (MQ=255)                                              gATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATc                                                                                                  >  2:619882/1‑104 (MQ=255)                                              gATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGa                                                                             >  2:312418/1‑125 (MQ=255)                                              gATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGa                                                                             <  1:312418/125‑1 (MQ=255)                                                tGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTCATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCa                                                             >  1:62350/1‑139 (MQ=255)                                                 gACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                                                            <  1:238831/139‑1 (MQ=255)                                                        cGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCa                                                     <  2:402536/139‑1 (MQ=255)                                                                 gCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGc                                            <  1:427899/139‑1 (MQ=255)                                                                          tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                                                            >  1:673585/1‑114 (MQ=255)                                                                          tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCAt                                                            <  2:673585/114‑1 (MQ=255)                                                                          tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGt                                                       <  1:519143/119‑1 (MQ=255)                                                                          tGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGt                                                       >  2:519143/1‑119 (MQ=255)                                                                           gCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTg                                  >  1:227196/1‑139 (MQ=255)                                                                            cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGc                                                                                                        >  2:559360/1‑68 (MQ=255)                                                                            cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGc                                                                                                        <  1:559360/68‑1 (MQ=255)                                                                            cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATa                                                                           >  2:579356/1‑97 (MQ=255)                                                                            cAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATa                                                                           <  1:579356/97‑1 (MQ=255)                                                                                tAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGa                             <  1:724382/139‑1 (MQ=255)                                                                                           cGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATAcg                                                                                        <  2:411541/69‑1 (MQ=255)                                                                                           cGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATAcg                                                                                        >  1:411541/1‑69 (MQ=255)                                                                                           cGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATa                  >  2:314642/1‑139 (MQ=255)                                                                                                             cGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTcc                                                              >  2:213217/1‑77 (MQ=255)                                                                                                             cGCCCTGCACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTcc                                                              <  1:213217/77‑1 (MQ=255)                                                                                                                    cACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGa                    >  1:913404/1‑112 (MQ=255)                                                                                                                    cACTTTCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGa                    <  2:913404/112‑1 (MQ=255)                                                                                                                         tCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAatgaatga  <  2:36020/125‑1 (MQ=255)                                                                                                                         tCAACGTCAGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAatgaatga  >  1:36020/1‑125 (MQ=255)                                                                                                                                 |                                                                                                                     GCTGTGGCAATTTCTGGCTACCATCCATCGCAATCTGCCAGGTTCGATGACGTAACGCCGGGTTGCTATAGCGTGCAATTAATCGGTTAGCGTAATCTTGCAAATCAACGCCCTGCACTTTCAACGTCGGCGCTTGTTCCTGCAACATCAAGCCATACGCCGCATAACGATAATGTTCATCTTCCATACAGTCATTAATGTGCTGATATCCTGCAAGATACCCCAGATACGCCAGGAATGAATGA  >  NC_000913/1629785‑1630029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑