Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,735,897	T→C	Y174H (TAT→CAT)	sodB →	superoxide dismutase, Fe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,735,897	0	T	C	100.0%	104.0 / NA	28	Y174H (TAT→CAT)	sodB	superoxide dismutase, Fe
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

CTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCAT  >  NC_000913/1735794‑1736020                                                                                                        |                                                                                                                            ctctAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGaa                                                                                          >  2:761082/1‑139 (MQ=255)                       accaccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                    >  2:800190/1‑139 (MQ=255)                         caccGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGTTGGGGAACGGGGAATCCGTAGCGAAAATTCTCGCTgaa                                                                  >  2:55923/1‑137 (MQ=255)                                  gACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTg                                                         <  1:761082/139‑1 (MQ=255)                                    cTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGAt                                                       >  1:233999/1‑139 (MQ=255)                                       cGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGc                                                    <  2:233999/139‑1 (MQ=255)                                                           tGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                    >  2:97106/1‑103 (MQ=255)                                                           tGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                    <  1:97106/103‑1 (MQ=255)                                                           tGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAg                                >  1:702906/1‑139 (MQ=255)                                                               aaCACGCTTATTACATCGACTATCGCAGTGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATGGCCTGAAGCGCt                            >  1:544259/1‑139 (MQ=255)                                                                  acGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAg                                                                                                                     >  1:732144/1‑47 (MQ=255)                                                                  acGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAg                                                                                                                     <  2:732144/47‑1 (MQ=255)                                                                   cGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                    >  2:847647/1‑95 (MQ=255)                                                                   cGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTg                                                                    <  1:847647/95‑1 (MQ=255)                                                                   cGCTTATTACATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGc                        >  1:911790/1‑139 (MQ=255)                                                                       tattaCATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt                    <  2:911790/139‑1 (MQ=255)                                                                            cATCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGc               <  2:912176/139‑1 (MQ=255)                                                                              tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCt             >  1:177824/1‑139 (MQ=255)                                                                              tCGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCt             >  2:893761/1‑139 (MQ=255)                                                                               cGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGc                                                    >  2:666319/1‑99 (MQ=255)                                                                               cGACTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGc                                                    <  1:666319/99‑1 (MQ=255)                                                                                 aCTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTAcg          >  2:493029/1‑139 (MQ=255)                                                                                 aCTATCGCAATGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTAcg          <  1:893761/139‑1 (MQ=255)                                                                                         aaTGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGCGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGatcat  <  2:544259/139‑1 (MQ=255)                                                                                           tGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATgcagca                                          <  2:890615/97‑1 (MQ=255)                                                                                           tGCACGTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATgcagca                                          >  1:890615/1‑97 (MQ=255)                                                                                                gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt                    <  1:390002/114‑1 (MQ=255)                                                                                                gTCCTGGCCATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTAt                    >  2:390002/1‑114 (MQ=255)                                                                                                        |                                                                                                                            CTCTAACGCGGGTACTCCGCTGACCACCGATGCGACTCCGCTGCTGACCGTTGATGTCTGGGAACACGCTTATTACATCGACTATCGCAATGCACGTCCTGGCTATCTGGAGCACTTCTGGGCGCTGGTGAACTGGGAATTCGTAGCGAAAAATCTCGCTGCATAATAACTGATGGCAAATGCAGCATTGCCTGAAGCGCTACGCTTATCAGGCCTACGCGGATCAT  >  NC_000913/1735794‑1736020

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑