Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,071,304	A→G	intergenic (+93/‑13)	yeeP → / → isrC	pseudogene, CP4‑44 prophage, 50S ribosome‑binding GTPase family,Phage or Prophage Related, putative histone/novel sRNA, CP4‑44, putative prophage remnant

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,071,304	0	A	G	100.0%	198.6 / NA	51	intergenic (+93/‑13)	yeeP/isrC	pseudogene, CP4‑44 prophage, 50S ribosome‑binding GTPase family,Phage or Prophage Related, putative histone/novel sRNA, CP4‑44, putative prophage remnant
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/26);  total (25/26)

CCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCACAATTCAGAAA  >  NC_000913/2071180‑2071438                                                                                                                             |                                                                                                                                       cccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                                          >  1:558433/1‑139 (MQ=255) cccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                                          >  2:515286/1‑139 (MQ=255)  ccGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         <  2:624074/139‑1 (MQ=255)               aaCTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGTACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTAtttt                                                                                                            >  2:280842/1‑139 (MQ=255)                   ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGTACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  1:280842/139‑1 (MQ=255)                   ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  1:515286/139‑1 (MQ=255)                   ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  1:374141/139‑1 (MQ=255)                   ggATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:558433/139‑1 (MQ=255)                    gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                                     >  2:439945/1‑125 (MQ=255)                    gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                                     <  1:439945/125‑1 (MQ=255)                    gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:431872/138‑1 (MQ=255)                    gATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  1:431872/1‑138 (MQ=255)                         tcttctGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAATCGATCAATATCTATTTTatcg                                                                                                        >  2:250095/1‑133 (MQ=255)                         tcttctGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAATCGATCAATATCTATTTTatcg                                                                                                        <  1:250095/133‑1 (MQ=255)                         tcttctGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGtt                                                                                                  >  2:388206/1‑139 (MQ=255)                                          gACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGc                                                                                 <  1:30307/139‑1 (MQ=255)                                               aTGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                                          <  1:91022/93‑1 (MQ=255)                                               aTGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGa                                                                                                                          >  2:91022/1‑93 (MQ=255)                                                tGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCtaataa                                                                           >  2:453865/1‑139 (MQ=255)                                                       cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         <  2:109776/86‑1 (MQ=255)                                                       cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGAt                                                                                                                         >  1:109776/1‑86 (MQ=255)                                                       cctGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCtt                                                                    >  2:375716/1‑139 (MQ=255)                                                         tGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                                     >  1:902391/1‑88 (MQ=255)                                                         tGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAat                                                                                                                     <  2:902391/88‑1 (MQ=255)                                                               tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                                                <  1:717967/119‑1 (MQ=255)                                                               tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCt                                                                                >  2:717967/1‑119 (MQ=255)                                                               tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                                               >  1:815995/1‑136 (MQ=255)                                                               tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCa                                                               <  2:815995/136‑1 (MQ=255)                                                               tGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTa                                                            <  2:443319/139‑1 (MQ=255)                                                                 aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTAtt                                                                                                              <  2:915246/87‑1 (MQ=255)                                                                 aGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTAtt                                                                                                              >  1:915246/1‑87 (MQ=255)                                                                     tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAg                                                                                  >  2:796855/1‑111 (MQ=255)                                                                     tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAg                                                                                  <  1:796855/111‑1 (MQ=255)                                                                     tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGc                                                      >  1:898943/1‑139 (MQ=255)                                                                     tGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGc                                                      >  2:514442/1‑139 (MQ=255)                                                                              aaGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACg                                             <  1:388206/139‑1 (MQ=255)                                                                                gCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTa                                           <  1:453865/139‑1 (MQ=255)                                                                                 cACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGa                                                 >  2:254544/1‑132 (MQ=255)                                                                                 cACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGa                                                 <  1:254544/132‑1 (MQ=255)                                                                                              aCTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        <  2:368284/64‑1 (MQ=255)                                                                                              aCTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTatcg                                                                                                        >  1:368284/1‑64 (MQ=255)                                                                                              aCTGTCTCTCTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCa                             <  1:375716/139‑1 (MQ=255)                                                                                                  tctctcTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCtt                                                                    >  1:579813/1‑96 (MQ=255)                                                                                                  tctctcTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCtt                                                                    <  2:579813/96‑1 (MQ=255)                                                                                                  tctctcTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGca                                                     >  2:478077/1‑111 (MQ=255)                                                                                                  tctctcTTGTCCGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGca                                                     <  1:478077/111‑1 (MQ=255)                                                                                                            ccGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAAc               <  1:514442/139‑1 (MQ=255)                                                                                                            ccGTGCAATAGCTCAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAAc               >  1:782748/1‑139 (MQ=255)                                                                                                                         cAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCa                             >  1:11514/1‑112 (MQ=255)                                                                                                                         cAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCa                             <  2:11514/112‑1 (MQ=255)                                                                                                                         cAATGATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCACAATTCAGaaa  >  1:210654/1‑139 (MQ=255)                                                                                                                             |                                                                                                                                       CCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAGCTCAATAATAGAATAAAACGATCAATATCTATTTTATCGATCGTTTATATCGATCGATAAGCTAATAATAACCTTTGTCAGTAACATGCACAGATACGTACAGAAAGACATTCAGGGAACAACAGAACCACAATTCAGAAA  >  NC_000913/2071180‑2071438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑