| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,073,282 | G→A | G582R (GGA→AGA) | flu → | CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,073,282 | 0 | G | A | 96.7% | 96.8 / ‑6.3 | 30 | G582R (GGA→AGA) | flu | CP4‑44 prophage, antigen 43 (Ag43) phase‑variable biofilm formation autotransporter |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (15/14); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGA > NC_000913/2073148‑2073416 | cccTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACa > 1:768175/1‑139 (MQ=255) aaGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGa > 2:685887/1‑132 (MQ=255) cGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCAcc < 1:753178/139‑1 (MQ=255) cTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc > 2:524915/1‑131 (MQ=255) cTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc < 1:524915/131‑1 (MQ=255) cGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCt > 2:509545/1‑139 (MQ=255) ccTCCGGTGCCACCTGGTATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCt > 1:522451/1‑139 (MQ=255) ccACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACa < 1:509545/139‑1 (MQ=255) ggAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg < 2:768175/139‑1 (MQ=255) ccccGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGAtt > 1:637188/1‑58 (MQ=255) ccccGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGAtt < 2:637188/58‑1 (MQ=255) ccccGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc < 1:294442/72‑1 (MQ=255) ccccGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCAc > 2:294442/1‑72 (MQ=255) ccACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCg > 1:51278/1‑64 (MQ=255) ccACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCg < 2:51278/64‑1 (MQ=255) ggtggtGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg < 2:249244/108‑1 (MQ=255) ggtggtGGATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg > 1:249244/1‑108 (MQ=255) ggtggATGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca > 1:403781/1‑139 (MQ=255) aTGACCTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGaa > 2:527859/1‑139 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCAGCATCAGCCTGCGTGTa > 2:184959/1‑115 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCt > 2:586797/1‑108 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCgtg < 1:94199/113‑1 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCgtg > 2:94199/1‑113 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTa < 1:184959/115‑1 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca > 2:462499/1‑130 (MQ=255) ccTCAGCCATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGca < 1:462499/130‑1 (MQ=255) ccTCAGCCATGCCACACAGATTCATTTCACCTGCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCt < 1:586797/108‑1 (MQ=255) ccATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg < 1:660803/90‑1 (MQ=255) ccATGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATgg > 2:660803/1‑90 (MQ=255) tGCCAGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGa < 2:403781/139‑1 (MQ=255) | CCCTGAAGCTGACCGGCAGCACTGTGCTGAACGGTGCCATTGACCCCACGAATGTCACTCTCGCCTCCGGTGCCACCTGGAATATCCCCGATAACGCCACGGTGCAGTCGGTGGTGGATGACCTCAGCCATGCCGGACAGATTCATTTCACCTCCACCCGCACAGGGAAGTTCGTACCGGCAACCCTGAAAGTGAAAAACCTGAACGGACAGAATGGCACCATCAGCCTGCGTGTACGCCCGGATATGGCACAGAACAATGCTGACAGA > NC_000913/2073148‑2073416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |