| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,184,452 | G→A | A22V (GCC→GTC) | thiD ← | hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,184,452 | 0 | G | A | 100.0% | 103.9 / NA | 32 | A22V (GCC→GTC) | thiD | hydroxy‑methylpyrimidine kinase and hydroxy‑phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/16); total (16/16) | |||||||||||
CGAATCGAGCTGGGCGGCGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGGCCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGAACAAAACT > NC_000913/2184319‑2184572 | cGAATCGAGCTGGGCGGCGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGa > 1:264393/1‑139 (MQ=255) aaTCGAGCTGGGCGGCGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAAt < 1:332982/139‑1 (MQ=255) aaTCGAGCTGGGCGGCGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAAt < 1:40892/139‑1 (MQ=255) tCGAGCTGGGCGGCGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATcc < 2:264393/139‑1 (MQ=255) ggcggcGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGcacca < 1:404081/139‑1 (MQ=255) cgACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt > 2:359613/1‑139 (MQ=255) cAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGt < 1:271466/139‑1 (MQ=255) cGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCg > 2:139474/1‑139 (MQ=255) cGAATGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCg < 2:589027/139‑1 (MQ=255) ccACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGCATCAGTACCGGCAATCCTCAGAGCGTTAATTCGtt > 1:729526/1‑139 (MQ=255) cgcCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTAc > 1:379359/1‑98 (MQ=255) cgcCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTAc < 2:379359/98‑1 (MQ=255) cgcCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCAcct > 2:847100/1‑139 (MQ=255) ccaccaGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGcc > 1:335197/1‑130 (MQ=255) ccaccaGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGcc < 2:335197/130‑1 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTg > 2:730378/1‑50 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTg < 1:730378/50‑1 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTg > 2:207415/1‑124 (MQ=255) aCTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTg < 1:207415/124‑1 (MQ=255) tGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTc > 1:44957/1‑126 (MQ=255) tGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTc < 2:44957/126‑1 (MQ=255) ccATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCa > 1:772834/1‑82 (MQ=255) ccATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCa < 2:772834/82‑1 (MQ=255) cATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAgg < 1:139474/139‑1 (MQ=255) aGCGCCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCACGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAAt > 1:432216/1‑139 (MQ=255) cgcCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGa < 1:642716/56‑1 (MQ=255) cgcCAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGa > 2:642716/1‑56 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt < 1:451542/49‑1 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACt > 2:451542/1‑49 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCa < 1:677423/90‑1 (MQ=255) cAAGTGCCGAGAAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCa > 2:677423/1‑90 (MQ=255) gagaAGGTTTTAAGATCGACCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGAACAAAACt > 1:348810/1‑139 (MQ=255) | CGAATCGAGCTGGGCGGCGACAAAATCAGGCTCAATGCGATACACCGACTGTACGCCACGGGTATTTTGCGCCACCAGTGCAGTAATAACTGAGCAACCATAAGCGCCAAGTGCCGAGAAGGTTTTAAGATCGGCCTGAATCCCCGCACCACCACTCGGATCAGTACCGGCAATCGTCAGAGCGTTAATTCGTTTCATGCCTGCACCTCCTGCGTCAATTGCCAGAGCGCATCAAGGAAATGTGGAACAAAACT > NC_000913/2184319‑2184572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |