Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,248,780	C→T	R280Q (CGG→CAG)	yeiE ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,248,780	0	C	T	100.0%	73.7 / NA	22	R280Q (CGG→CAG)	yeiE	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/8);  total (14/8)

GGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAC  >  NC_000913/2248669‑2248908                                                                                                                |                                                                                                                                 ggCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTgg                                                                                                                      <  1:28853/124‑1 (MQ=255) ggCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTgg                                                                                                                      >  2:28853/1‑124 (MQ=255)  gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                      >  2:826876/1‑139 (MQ=255)  gCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                      >  2:31602/1‑139 (MQ=255)                   gcgcTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCcaca                                                                                     >  1:214615/1‑139 (MQ=255)                       tGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGt                                                                                 >  2:298345/1‑139 (MQ=255)                              cGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATc                                                                          <  1:31602/139‑1 (MQ=255)                                            cACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGC‑GGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                   <  2:778992/99‑1 (MQ=255)                                            cACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGC‑GGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCgatg                                                                                                   >  1:778992/1‑99 (MQ=255)                                             aCCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCAt                                                                           >  1:251107/1‑123 (MQ=255)                                             aCCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCAt                                                                           <  2:251107/123‑1 (MQ=255)                                                    tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                      <  2:276088/89‑1 (MQ=255)                                                    tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCg                                                                                                      >  1:276088/1‑89 (MQ=255)                                                    tGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCACAGGGACCGCaa                                                    >  2:591143/1‑139 (MQ=255)                                                      ttCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACt                                                  >  2:497536/1‑139 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTa                                                                                                                               >  2:293737/1‑49 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTa                                                                                                                               <  1:293737/49‑1 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAgg                                                                       >  1:39956/1‑105 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAgg                                                                       <  2:39956/105‑1 (MQ=255)                                                                   aCTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGtgc                                     >  1:808139/1‑139 (MQ=255)                                                                         cgcgGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCAATTAATGTgcctgcct                               <  1:497536/139‑1 (MQ=255)                                                                                                      cAGAAAGCGCTGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAc  >  2:738255/1‑139 (MQ=255)                                                                                                                |                                                                                                                                 GGCGAATGCATGCTGAAGGCGCTGCGTCACGAAATCGACACAGCACCAGCATGTTCTTGTACAGCAACTTAACGCGGCACATTTGCGGGATCGCAATAGTCCAGAAAGCGCCGTAGCGCGTTGGAAAGGTGTTTTTGCCGATGATGTATACGCCACAACGTACGCATCAGGCGCGGCAGAGGGACCGCAACTTCACTTAATGTGCCTGCCTGCAATTGATCTTCAATCACACGTCGCGAC  >  NC_000913/2248669‑2248908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑