Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,846,715	T→C	Q233R (CAG→CGG)	hycD ←	hydrogenase 3, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,846,715	0	T	C	100.0%	75.8 / NA	21	Q233R (CAG→CGG)	hycD	hydrogenase 3, membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/9);  total (12/9)

TGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGCCCATT  >  NC_000913/2846581‑2846846                                                                                                                                       |                                                                                                                                    tGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCaa                                                                                                                                 <  2:761572/139‑1 (MQ=255)                        aCCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGa                                                                                                          <  2:597853/139‑1 (MQ=255)                           aGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATAc                                                                                                       <  2:287220/139‑1 (MQ=255)                                  cGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCAttt                                                                                                >  1:752981/1‑139 (MQ=255)                                                      gCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAgccgctgc                                                                            >  1:444121/1‑139 (MQ=255)                                                           cACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGccgctgccgctg                                                                       <  2:752981/139‑1 (MQ=255)                                                                                  cggcggTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTg                                                >  1:899676/1‑139 (MQ=255)                                                                                       gTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCagaga                                                             <  1:463993/121‑1 (MQ=255)                                                                                       gTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCagaga                                                             >  2:463993/1‑121 (MQ=255)                                                                                                       gTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATAc                                                                                                       >  2:763674/1‑63 (MQ=255)                                                                                                       gTCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATAc                                                                                                       <  1:763674/63‑1 (MQ=255)                                                                                                        tCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAg                                                                                                                        <  1:723608/45‑1 (MQ=255)                                                                                                        tCCCCACGGAATAAACACCCCGACGAACATCCGCAACACCACCAg                                                                                                                        >  2:723608/1‑45 (MQ=255)                                                                                                                   tAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAGgccgctgccgc                                                                         <  1:245830/81‑1 (MQ=255)                                                                                                                   tAAACACCCCGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAGgccgctgccgc                                                                         >  2:245830/1‑81 (MQ=255)                                                                                                                           ccGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGc       >  2:151620/1‑139 (MQ=255)                                                                                                                           ccGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGc       >  2:474407/1‑139 (MQ=255)                                                                                                                           ccGACGAACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGc       >  2:382242/1‑139 (MQ=255)                                                                                                                               cgaACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGCCCAt   >  1:58288/1‑139 (MQ=255)                                                                                                                               cgaACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGCCCAt   >  1:341405/1‑139 (MQ=255)                                                                                                                                gaACATCCGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGCCCAtt  <  1:756496/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACCGGCGGTGAAGGTTTCCATTTGTCCCCACGGAATAAACACCCCGACGAACATCTGCAACACCACCAGCTGTTTCAGGCTGATACCCCATTTCATGACGCCAAAGCCGCTGCCGCTGTATTCAGAGAGCGGGCCTTCCTGCAGCTCCTGCTCGGCTTCCGCCAGGTCGAACGGCAGTTTGCCCATT  >  NC_000913/2846581‑2846846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑