Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,271	A→G	intergenic (‑147/+140)	kduI ← / ← yqeF	hexuronate isomerase/short chain acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,271	0	A	G	100.0%	121.8 / NA	33	intergenic (‑147/+140)	kduI/yqeF	hexuronate isomerase/short chain acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/16);  total (17/16)

GATAATCAGTGAAGCGTGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGC  >  NC_000913/2984135‑2984406                                                                                                                                         |                                                                                                                                        gATAATCAGTGAAGCGTGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTaagaa                                                                                                                                       <  2:575145/139‑1 (MQ=255)        aGTGAAGCGTGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCa                                                                                                                                <  1:689751/139‑1 (MQ=255)           gAAGCGTGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAgtg                                                                                                                             <  2:205782/139‑1 (MQ=255)                gTGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGAt                                                                                                                        <  1:665916/139‑1 (MQ=255)                 tGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGATAAGGATTAAGAAAAGTGCAGTGTCGATc                                                                                                                       >  2:506095/1‑139 (MQ=255)                         gAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAatat                                                                                                               <  1:863038/139‑1 (MQ=255)                                                   ttttCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAg                                                                                     <  2:599544/139‑1 (MQ=255)                                                                    tgatATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGaa                                                                                                                  <  2:861029/93‑1 (MQ=255)                                                                    tgatATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGaa                                                                                                                  >  1:861029/1‑93 (MQ=255)                                                                      atatGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTg                                                                                                                                  <  1:639785/75‑1 (MQ=255)                                                                      atatGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTg                                                                                                                                  >  2:639785/1‑75 (MQ=255)                                                                          gCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTg                                                              >  1:108841/1‑139 (MQ=255)                                                                                      gggTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGcc                                                                                   <  1:405172/106‑1 (MQ=255)                                                                                      gggTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGcc                                                                                   >  2:405172/1‑106 (MQ=255)                                                                                          tttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTgccagc                                                         >  2:591647/1‑128 (MQ=255)                                                                                          tttCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTgccagc                                                         <  1:591647/128‑1 (MQ=255)                                                                                            tCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTaagaa                                                                                                                                       >  1:512568/1‑48 (MQ=255)                                                                                            tCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTaagaa                                                                                                                                       <  2:512568/48‑1 (MQ=255)                                                                                                 tAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAg                                                                                >  1:48543/1‑98 (MQ=255)                                                                                                 tAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAg                                                                                <  2:48543/98‑1 (MQ=255)                                                                                                   aaaaTAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTgccagc                                                         <  2:841534/119‑1 (MQ=255)                                                                                                   aaaaTAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTgccagc                                                         >  1:841534/1‑119 (MQ=255)                                                                                                   aaaaTAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCcat                                                      >  1:508351/1‑122 (MQ=255)                                                                                                   aaaaTAAAACAATGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCcat                                                      <  2:508351/122‑1 (MQ=255)                                                                                                               tGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCAt                                                                                                          >  2:669577/1‑58 (MQ=255)                                                                                                               tGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCAt                                                                                                          <  1:669577/58‑1 (MQ=255)                                                                                                               tGTTTCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATTTAGGAGAACTGGCAGGCCTCAATTATTCAACA‑ATTaaataagggaaaaa                        >  2:130512/1‑127 (MQ=255)                                                                                                                 tttCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGatat                       >  2:60701/1‑139 (MQ=255)                                                                                                                 tttCATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGatat                       >  2:790866/1‑139 (MQ=255)                                                                                                                    cATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGc                                                                                    >  2:61757/1‑75 (MQ=255)                                                                                                                    cATTTTTAAGTTAAGGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGc                                                                                    <  1:61757/75‑1 (MQ=255)                                                                                                                               taagGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCcat                                                      >  1:851689/1‑94 (MQ=255)                                                                                                                               taagGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCcat                                                      <  2:851689/94‑1 (MQ=255)                                                                                                                                 agGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACgg                              <  2:323954/116‑1 (MQ=255)                                                                                                                                 agGATTAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACgg                              >  1:323954/1‑116 (MQ=255)                                                                                                                                      tAAGAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGc  >  2:704318/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                        GATAATCAGTGAAGCGTGAAAATAGAAAACGATAGTGAAACACTGTTTCGTTTTCTCTATAAAGGGATGATATGCCCATGGGCTAGGGTTTTCAATTAAAAATAAAACAATGTTTCATTTTTAAGTTAAGGATTAAAAAAAGTGCAGTGTCGATCACGAATATGTCATGAGAATGGAAAAATGAGGGAGCCCAGAAAATGTATGTCGACTTGCCAGCCATCATTATTCAACAGATTAATGACGGCAGATATTTATCGGTAAGGAGGAAATGC  >  NC_000913/2984135‑2984406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑