Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 459,628 G→A A247A (GCG→GCA)  lon → DNA‑binding ATP‑dependent protease La

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913459,6280GA100.0% 84.5 / NA 25A247A (GCG→GCA) lonDNA‑binding ATP‑dependent protease La
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/11);  total (14/11)

CTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGA  >  NC_000913/459503‑459747
                                                                                                                             |                                                                                                                       
ctgctgCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAGAACg                                                                                                            <  1:746874/139‑1 (MQ=255)
             agaAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAgcg                                                                                               >  2:386965/1‑139 (MQ=255)
                        cGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGAcg                                                                                    >  2:819577/1‑139 (MQ=255)
                              cgcgTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGa                                                                              >  1:589489/1‑139 (MQ=255)
                                             aTGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAgg                                                               >  2:441867/1‑139 (MQ=255)
                                                           gCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg                                                                         >  1:400863/1‑115 (MQ=255)
                                                           gCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATg                                                                         <  2:400863/115‑1 (MQ=255)
                                                                  tACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCag                                          >  1:889329/1‑139 (MQ=255)
                                                                      aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGATGAAAACGAAGCCCTg                                                                                                    <  1:610665/77‑1 (MQ=255)
                                                                      aTCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCATg                                                                                                    >  2:610665/1‑77 (MQ=255)
                                                                            aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCa                                                             >  1:440857/1‑110 (MQ=255)
                                                                            aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCa                                                             <  2:440857/110‑1 (MQ=255)
                                                                            aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAagag                                                       <  1:101471/116‑1 (MQ=255)
                                                                            aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAagag                                                       >  2:101471/1‑116 (MQ=255)
                                                                            aCGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGaa                                >  1:686372/1‑139 (MQ=255)
                                                                               aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAg                                                                                                         <  2:571031/63‑1 (MQ=255)
                                                                               aGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAg                                                                                                         >  1:571031/1‑63 (MQ=255)
                                                                                              ttCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGa                                                                                      >  2:423603/1‑67 (MQ=255)
                                                                                              ttCAGAAAGAACTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGa                                                                                      <  1:423603/67‑1 (MQ=255)
                                                                                                 agaaagaaCTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAACGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGAt           <  2:656016/139‑1 (MQ=255)
                                                                                                   aaagaaCTCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGt         >  1:782271/1‑139 (MQ=255)
                                                                                                        aCTCGGTGAAATGGACGACGCACCGGACGAAAACg                                                                                                            <  2:800730/35‑1 (MQ=255)
                                                                                                        aCTCGGTGAAATGGACGACGCACCGGACGAAAACg                                                                                                            >  1:800730/1‑35 (MQ=255)
                                                                                                          tCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGa  <  1:215285/139‑1 (MQ=255)
                                                                                                          tCGGTGAAATGGACGACGCACCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGa  <  1:197704/139‑1 (MQ=255)
                                                                                                                             |                                                                                                                       
CTGCTGCAGGTTGAGAAACGCATTCGCAACCGCGTTAAAAAGCAGATGGAGAAATCCCAGCGTGAGTACTATCTGAACGAGCAAATGAAAGCTATTCAGAAAGAACTCGGTGAAATGGACGACGCGCCGGACGAAAACGAAGCCCTGAAGCGCAAAATCGACGCGGCGAAGATGCCGAAAGAGGCAAAAGAGAAAGCGGAAGCAGAGTTGCAGAAGCTGAAAATGATGTCTCCGATGTCGGCAGA  >  NC_000913/459503‑459747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑