Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,366,953	G→A	G10R (GGA→AGA)	yhcF →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,366,953	0	G	A	100.0%	206.6 / NA	59	G10R (GGA→AGA)	yhcF	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/28);  total (31/28)

TTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366826‑3367087                                                                                                                                |                                                                                                                                       ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                             <  1:875754/139‑1 (MQ=255) ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                             >  1:660626/1‑139 (MQ=255) ttAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCttt                                                                                                                             <  1:301267/139‑1 (MQ=255)  tAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCtttt                                                                                                                            >  2:675081/1‑139 (MQ=255)                   tGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           >  2:163894/1‑139 (MQ=255)                   tGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           >  2:536563/1‑139 (MQ=255)                     gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  1:479988/139‑1 (MQ=255)                     gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:410003/139‑1 (MQ=255)                     gCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:314514/1‑139 (MQ=255)                         cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGcc                                                                                                                       <  1:213533/121‑1 (MQ=255)                         cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGcc                                                                                                                       >  2:213533/1‑121 (MQ=255)                         cAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCt                                                                                                     >  2:407759/1‑139 (MQ=255)                               ttAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATaga                                                                                               <  1:407759/139‑1 (MQ=255)                                   ggATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTAt                                                                                           <  2:138640/139‑1 (MQ=255)                                      tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATag                                                                                                <  1:312230/131‑1 (MQ=255)                                      tGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATag                                                                                                >  2:312230/1‑131 (MQ=255)                                              aaCTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGtt                                                                                >  1:486174/1‑139 (MQ=255)                                                  aaCCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAgg                                                                            <  1:163894/139‑1 (MQ=255)                                                      gACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGAc                                                                        >  2:437195/1‑139 (MQ=255)                                                            aCAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGa                                                                  <  1:613638/139‑1 (MQ=255)                                                             cAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGaa                                                                 >  1:610593/1‑139 (MQ=255)                                                                   ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  <  2:66841/100‑1 (MQ=255)                                                                   ttACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGAt                                                                                                  >  1:66841/1‑100 (MQ=255)                                                                        ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  1:536563/139‑1 (MQ=255)                                                                        ggCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:418504/139‑1 (MQ=255)                                                                          cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         >  1:839109/1‑86 (MQ=255)                                                                          cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGc                                                                                                         <  2:839109/86‑1 (MQ=255)                                                                          cAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTa                                                    >  2:811989/1‑139 (MQ=255)                                                                           aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       <  1:147686/103‑1 (MQ=255)                                                                           aaTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAAt                                                                                       >  2:147686/1‑103 (MQ=255)                                                                            aTCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACt                                                  >  2:651063/1‑139 (MQ=255)                                                                             tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           >  2:59674/1‑81 (MQ=255)                                                                             tCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCaa                                                                                                           <  1:59674/81‑1 (MQ=255)                                                                                   ggTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATAc                                           >  1:296154/1‑139 (MQ=255)                                                                                       cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                       >  1:838877/1‑139 (MQ=255)                                                                                       cTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAg                                       <  2:296154/139‑1 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                                 <  1:506662/73‑1 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATa                                                                                                 >  2:506662/1‑73 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTg                                                                          <  1:582340/96‑1 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTg                                                                          >  2:582340/1‑96 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGTAGCTTGTACTCCAATACTAAGCAAcggcg                               <  2:610593/139‑1 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      >  1:192160/1‑116 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:192160/116‑1 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               <  1:757964/139‑1 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:347932/1‑139 (MQ=255)                                                                                               ggCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAAcggcg                               >  1:196334/1‑139 (MQ=255)                                                                                                gCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      >  1:746611/1‑115 (MQ=255)                                                                                                gCAAAATGAACAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:746611/115‑1 (MQ=255)                                                                                                          cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCaa                                    <  1:156857/123‑1 (MQ=255)                                                                                                          cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCaa                                    >  2:629515/1‑123 (MQ=255)                                                                                                          cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCaa                                    >  2:156857/1‑123 (MQ=255)                                                                                                          cAATGTAAAATTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCaa                                    <  1:629515/123‑1 (MQ=255)                                                                                                                 aaaTTACTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTa             >  2:372899/1‑139 (MQ=255)                                                                                                                      aCTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCAt        <  2:838877/139‑1 (MQ=255)                                                                                                                       cTGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCAGTAGTCAtt       >  1:537451/1‑139 (MQ=255)                                                                                                                        tGATTGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTc      <  1:651063/139‑1 (MQ=255)                                                                                                                            tGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      >  1:299439/1‑87 (MQ=255)                                                                                                                            tGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTg                                                      <  2:299439/87‑1 (MQ=255)                                                                                                                            tGCCAGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTc  >  2:194624/1‑139 (MQ=255)                                                                                                                                |                                                                                                                                       TTAGCCTCACACTGACAATGGCTCCAGCTTTTAAGGATGAAGGGGAACTAACCGACATGACAGATATTACAGGCAATCTGACGGTCCTGGTGGAGGCAAAATGAACAATGTAAAATTACTGATTGCCGGAAGTGCCTTTTTTGCCATGTCAGCGCAAGCCGCTGATAGAGTATCAATTGACGTTAAGGTGACTCTGGAAGCTGCAGCTTGTACTCCAATACTAAGCAACGGCGGAGTTGTTAACTTCGGTAGTCATTCAGTC  >  NC_000913/3366826‑3367087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑