Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,936,381 C→T T35M (ACG→ATG)  rbsB → D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,936,3810CT100.0% 115.0 / NA 34T35M (ACG→ATG) rbsBD‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/16);  total (18/16)

TACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACG  >  NC_000913/3936261‑3936500
                                                                                                                        |                                                                                                                       
tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCtt                                                                                                       >  2:471/1‑139 (MQ=255)
tACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCtt                                                                                                       >  2:777658/1‑139 (MQ=255)
  cAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTg                                                                                                     >  2:538438/1‑139 (MQ=255)
    ggACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTa                                                                                                   >  2:343062/1‑139 (MQ=255)
          cTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCCGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTg                                                                                             >  1:473924/1‑139 (MQ=255)
                    aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCg                                                                                                            >  1:46645/1‑114 (MQ=255)
                    aaCATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCg                                                                                                            <  2:46645/114‑1 (MQ=255)
                      cATGAAAAACCTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCAAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                                                                 <  1:538438/139‑1 (MQ=255)
                      cATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                                                                 <  2:473924/139‑1 (MQ=255)
                                          tGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACt                                                             <  2:889970/139‑1 (MQ=255)
                                           ggTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                                                                 <  2:533514/118‑1 (MQ=255)
                                           ggTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGgcgcg                                                                                 >  1:533514/1‑118 (MQ=255)
                                           ggTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACtt                                                            <  1:777658/139‑1 (MQ=255)
                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                                               >  1:783288/1‑86 (MQ=255)
                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTaacaac                                                                                                               <  2:783288/86‑1 (MQ=255)
                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCg                                                                                                <  1:823632/101‑1 (MQ=255)
                                             tttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCg                                                                                                >  2:823632/1‑101 (MQ=255)
                                              ttCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGc                                                         <  1:471/139‑1 (MQ=255)
                                                 cGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCtat                                                      >  2:669664/1‑139 (MQ=255)
                                                 cGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCtat                                                      >  2:527177/1‑139 (MQ=255)
                                                      ttGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCt                                                 >  2:508290/1‑139 (MQ=255)
                                                        gcgcTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGTAAGATGGCGCGCAGACAGAGGCGGATAAACTTGGCTATAACCtgg                                               <  1:527177/139‑1 (MQ=255)
                                                        gcgcTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg                                               <  1:343062/139‑1 (MQ=255)
                                                                        tCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAAc                                                   <  2:311729/119‑1 (MQ=255)
                                                                        tCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAAc                                                   >  1:311729/1‑119 (MQ=255)
                                                                                           aaaaGACACCATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAagag            <  1:669664/139‑1 (MQ=255)
                                                                                                   ccATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGaa    >  1:264616/1‑139 (MQ=255)
                                                                                                    cATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg                                               >  2:912086/1‑95 (MQ=255)
                                                                                                    cATCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCtgg                                               <  1:912086/95‑1 (MQ=255)
                                                                                                     aTCGCGCTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACg  >  1:28678/1‑139 (MQ=255)
                                                                                                         cgcTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAagag                                                                        <  2:319463/65‑1 (MQ=255)
                                                                                                         cgcTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAagag                                                                        >  1:319463/1‑65 (MQ=255)
                                                                                                          gcTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGa                                                                             <  2:188605/59‑1 (MQ=255)
                                                                                                          gcTGGTGGTCTCCATGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGa                                                                             >  1:188605/1‑59 (MQ=255)
                                                                                                                        |                                                                                                                       
TACAGGACATCTTGAATATGAACATGAAAAAACTGGCTACCCTGGTTTCCGCTGTTGCGCTAAGCGCCACCGTCAGTGCGAATGCGATGGCAAAAGACACCATCGCGCTGGTGGTCTCCACGCTTAACAACCCGTTCTTTGTATCGCTGAAAGATGGCGCGCAGAAAGAGGCGGATAAACTTGGCTATAACCTGGTGGTGCTGGACTCCCAGAACAACCCGGCGAAAGAGCTGGCGAACG  >  NC_000913/3936261‑3936500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: