Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,351,093	G→A	G190G (GGC→GGT)	dcuS ←	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,351,093	0	G	A	100.0%	131.6 / NA	38	G190G (GGC→GGT)	dcuS	sensory histidine kinase in two‑component regulatory system with DcuR, regulator of anaerobic fumarate respiration
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

CGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATG  >  NC_000913/4350960‑4351210                                                                                                                                      |                                                                                                                      cgCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTTCCAATCAGTCCGACCAGCATACCaaa                                                                                                                  <  1:671743/139‑1 (MQ=255) cgCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCaaa                                                                                                                  <  2:45777/139‑1 (MQ=255)   ccTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAaata                                                                                                                >  2:589003/1‑139 (MQ=255)     ttcttTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAaataat                                                                                                              >  1:377095/1‑139 (MQ=255)       cttTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATAc                                                                                                            >  1:554978/1‑139 (MQ=255)           gATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGAc                                                                                                        <  2:377095/139‑1 (MQ=255)                  tGCAACATGGCCTGGCGTTGTTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAAGAATACCGACCAGa                                                                                                    >  2:620419/1‑136 (MQ=255)                  tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGa                                                                                                    <  1:620419/136‑1 (MQ=255)                  tGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATaa                                                                                                 >  1:697136/1‑139 (MQ=255)                              tGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                     <  1:276882/139‑1 (MQ=255)                              tGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                     <  2:877303/139‑1 (MQ=255)                                     gCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCAtt                                                                              >  2:695123/1‑139 (MQ=255)                                      cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                     >  1:493588/1‑131 (MQ=255)                                      cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                     <  2:493588/131‑1 (MQ=255)                                      cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTg                                                                             >  1:611427/1‑139 (MQ=255)                                      cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTg                                                                             >  2:834262/1‑139 (MQ=255)                                      cTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTg                                                                             <  2:554978/139‑1 (MQ=255)                                                gTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                     >  2:23589/1‑121 (MQ=255)                                                gTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGAc                                                                                     <  1:23589/121‑1 (MQ=255)                                                gTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTggg                                                                   >  1:226756/1‑139 (MQ=255)                                                     gaTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTGCCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGTCCAGACAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                              <  2:611427/139‑1 (MQ=255)                                                     gaTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                              <  1:695123/139‑1 (MQ=255)                                                     gaTTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                              <  1:589003/139‑1 (MQ=255)                                                            tAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTa                                                       >  2:883577/1‑139 (MQ=255)                                                                      ggCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                              >  2:633446/1‑122 (MQ=255)                                                                      ggCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTcaca                                                              <  1:633446/122‑1 (MQ=255)                                                                               ggATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGccac                                    <  2:226756/139‑1 (MQ=255)                                                                                gATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGccacc                                   <  2:697136/139‑1 (MQ=255)                                                                                                             aGGTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTa      >  2:814175/1‑139 (MQ=255)                                                                                                               gTGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGa    <  1:834262/139‑1 (MQ=255)                                                                                                                tGCCAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGAt   <  1:883577/139‑1 (MQ=255)                                                                                                                  ccAATCAGTCCGACCAGCATACCAAATAATACCGAc                                                                                                        >  2:318560/1‑36 (MQ=255)                                                                                                                  ccAATCAGTCCGACCAGCATACCAAATAATACCGAc                                                                                                        <  1:318560/36‑1 (MQ=255)                                                                                                                  ccAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                      <  2:360769/54‑1 (MQ=255)                                                                                                                  ccAATCAGTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGa                                                                                      >  1:360769/1‑54 (MQ=255)                                                                                                                         gTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATTGTAGATg  <  2:187935/131‑1 (MQ=255)                                                                                                                         gTCCGACCAGCATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATTGTAGATg  >  1:187935/1‑131 (MQ=255)                                                                                                                                   cATACCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTca                                                                <  2:360072/59‑1 (MQ=255)                                                                                                                                      |                                                                                                                      CGCCTTCTTTGATAGACTGCAACATGGCCTGGCGTTGCTCAAACAGCGTGGAGATTTCGTAGGGTTCCAGGCCGAAAAGGATTTTTTTCAGTACCTTAACCAGAATGCAGGTGCCAATCAGTCCGACCAGCATGCCAAATAATACCGACCAGATAATGCTCCAGCGACTGTCATTGATCTGTTGGGTCACACGGCTTAACTCAAGGCCGATCGCCACCACGCCAATTTGTTTATGATTTTCATCGTAGATG  >  NC_000913/4350960‑4351210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑