Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,574,259	A→G	pseudogene (341/2937 nt)	yjiV →	pseudogene, conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,574,259	0	A	G	100.0%	137.7 / NA	36	pseudogene (341/2937 nt)	yjiV	pseudogene, conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/19);  total (17/19)

TATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAGG  >  NC_000913/4574132‑4574394                                                                                                                                |                                                                                                                                        tatgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTg                                                                                                                              <  1:332212/139‑1 (MQ=255)   tgtgtatgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGc                                                                                                                            <  1:641338/139‑1 (MQ=255)         tgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa                                                                                                                      >  2:300025/1‑139 (MQ=255)         tgtgAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa                                                                                                                      >  1:418769/1‑139 (MQ=255)             aaCCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATtct                                                                                                                  >  1:524085/1‑139 (MQ=255)                 aGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCttt                                                                                                              >  1:340622/1‑139 (MQ=255)                 aGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCttt                                                                                                              >  2:559363/1‑139 (MQ=255)                  gCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTg                                                                                                             <  1:559363/139‑1 (MQ=255)                               gACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTAc                                                                                                <  1:556427/139‑1 (MQ=255)                               gACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTAc                                                                                                <  1:573237/139‑1 (MQ=255)                                        aGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAAc                                                                                       <  2:305853/139‑1 (MQ=255)                                                         tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAgg                                                                                                                                >  2:4869/1‑81 (MQ=255)                                                         tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAgg                                                                                                                                <  1:4869/81‑1 (MQ=255)                                                         tGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAgaagaa                                                                      >  2:870188/1‑139 (MQ=255)                                                                tGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACg                                                                                 <  1:895152/121‑1 (MQ=255)                                                                tGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACg                                                                                 >  2:895152/1‑121 (MQ=255)                                                                tGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCg                                                               >  2:25711/1‑139 (MQ=255)                                                                  tAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGaa                                                             >  1:462635/1‑139 (MQ=255)                                                                          gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAgg                                                                                                         <  2:885007/87‑1 (MQ=255)                                                                          gTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAgg                                                                                                         >  1:885007/1‑87 (MQ=255)                                                                           tAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTATAGAAGAAAAAGTCGAAAAAGAGAAc                                                    <  1:127349/139‑1 (MQ=255)                                                                            aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACa                                                                                      >  2:128045/1‑104 (MQ=255)                                                                            aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACa                                                                                      <  1:128045/104‑1 (MQ=255)                                                                            aaTGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACa                                                   >  1:79929/1‑139 (MQ=255)                                                                                  ccTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGa                                                                                   <  2:893005/101‑1 (MQ=255)                                                                                  ccTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGa                                                                                   >  1:893005/1‑101 (MQ=255)                                                                                  ccTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTc                                             >  1:393652/1‑139 (MQ=255)                                                                                       tcttctGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCt                                        <  1:300025/139‑1 (MQ=255)                                                                                          tctGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCg                                     <  2:418769/139‑1 (MQ=255)                                                                                            tGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa                                                                                                                      <  2:295489/56‑1 (MQ=255)                                                                                            tGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGaa                                                                                                                      >  1:295489/1‑56 (MQ=255)                                                                                                            gggCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGga                   <  2:524085/139‑1 (MQ=255)                                                                                                              gCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGaa                                                                                                    >  1:788289/1‑56 (MQ=255)                                                                                                              gCAAACTAGTCCAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGaa                                                                                                    <  2:788289/56‑1 (MQ=255)                                                                                                                        ccAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAg       <  2:462635/139‑1 (MQ=255)                                                                                                                         cAGTGGGGAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAgg      <  2:340622/139‑1 (MQ=255)                                                                                                                             ggggAAATAAAGGTGGCGATGAATTCTCTTTGCATGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGTCGCAGGAGAGACCAGCGAGGCAgg  <  1:870188/139‑1 (MQ=255)                                                                                                                                |                                                                                                                                        TATGTGTATGTGAACCAGCGACAAAATTGTGACTGTGGAAGCCCTGTATACGAAGTTGCATTCTGTAATGATTGTAATGAGCCTCATCTTCTGGCACGGGACAAAAAGGGCAAACTAGTCCAGTGGGAAAATAAAGGTGGCGATGAATTCTCTTTGCAGGATGAAGTACCTGTTGAACATGACGCTACAGAAGAAAAAGTCGAAAAAGAGAACAGTTTTCAGCCTCCGTTGATTATTGCCGCAGGAGAGACCAGCGAGGCAGG  >  NC_000913/4574132‑4574394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑