Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	731,913	A→G	A777A (GCA→GCG)	rhsC →	Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	731,913	0	A	G	100.0%	25.9 / NA	8	A777A (GCA→GCG)	rhsC	Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (2/6);  total (2/6)

TGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCG  >  NC_000913/731876‑732034                                      |                                                                                                                          tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGaaa                                    >  1:37626/1‑125 (MQ=255) tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGaaa                                    <  2:37626/125‑1 (MQ=255) tGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACAcc                      <  2:911089/139‑1 (MQ=255)   ctctGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGGTGACCTACGTCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGc                    <  1:561526/139‑1 (MQ=255)   ctctGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGc                    <  2:532423/139‑1 (MQ=255)   ctctGGCAGCATGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGc                    >  2:822520/1‑139 (MQ=255)            caTGAGACCAGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGt           <  1:688043/139‑1 (MQ=255)                     aGACATGCGTACAACGCGCAGGGGCTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCcgcg  <  2:40530/139‑1 (MQ=255)                                      |                                                                                                                          TGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCTGGTGGAGTACACCCGCG  >  NC_000913/731876‑732034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑