Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,620,907	(A)8→7	intergenic (‑35/‑290)	upp ← / → purM	uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,620,900	0	A	.	100.0%	100.3 / NA	24	intergenic (‑28/‑297)	upp/purM	uracil phosphoribosyltransferase/phosphoribosylaminoimidazole synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (10/14);  total (10/14)

ACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGAAA  >  NC_000913/2620769‑2621017                                                                                                                                    |                                                                                                                      aCTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACaaaaaaat                                                                                                                >  2:492147/1‑138 (MQ=255)       gAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACgg                                                                                                         <  1:569526/139‑1 (MQ=255)       gAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACgg                                                                                                         <  2:279692/139‑1 (MQ=255)            gAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATtat                                                                                                    <  1:492147/139‑1 (MQ=255)              gTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATAc                                                                                                  <  1:481449/139‑1 (MQ=255)                                     atatCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAata                                                                           >  1:520522/1‑139 (MQ=255)                                          ttGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCtt                                                                      >  1:249313/1‑139 (MQ=255)                                             cTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTc                                                                   >  2:294135/1‑139 (MQ=255)                                                         tCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGt                                                       <  1:294135/139‑1 (MQ=255)                                                                   tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGt                                                       <  1:447243/129‑1 (MQ=255)                                                                   tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGt                                                       >  2:447243/1‑129 (MQ=255)                                                                   tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa                                             >  1:20188/1‑139 (MQ=255)                                                                   tgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATa                                             <  2:20188/139‑1 (MQ=255)                                                                                    tttGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGt                            >  1:121280/1‑139 (MQ=255)                                                                                     ttGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAACAATTCGACGGATTATACCTCATTTCTGCTAGGCGGCACTATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGtt                           <  1:159250/139‑1 (MQ=255)                                                                                              aCGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTatat                  <  2:249313/139‑1 (MQ=255)                                                                                                             tttCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAg                             <  1:512272/113‑1 (MQ=255)                                                                                                             tttCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAg                             >  2:512272/1‑113 (MQ=255)                                                                                                              ttCTCCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa  <  2:121280/139‑1 (MQ=255)                                                                                                               tctcCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa  >  2:201899/1‑138 (MQ=255)                                                                                                               tctcCTTTGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa  <  1:201899/138‑1 (MQ=255)                                                                                                                     ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAg                                                                                    <  1:79621/50‑1 (MQ=255)                                                                                                                     ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAg                                                                                    >  2:79621/1‑50 (MQ=255)                                                                                                                     ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa  <  1:516763/132‑1 (MQ=255)                                                                                                                     ttGAGGGGCAGCCAC‑AAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGaaa  >  2:516763/1‑132 (MQ=255)                                                                                                                                    |                                                                                                                      ACTTCGGAAGCGAGTTCGCGAAAGCGCTTGGTGCTGATATCTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAAATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTTGAGATAAAGTTATTTTATATTCAGATGGTTATGAAA  >  NC_000913/2620769‑2621017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑