Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,468 (G)5→4 intergenic (+48/‑39) ilvL → / → ilvX ilvG operon leader peptide/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,4640G.100.0% 96.3 / NA 23intergenic (+44/‑43)ilvL/ilvXilvG operon leader peptide/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (12/11);  total (12/11)

GATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGC  >  NC_000913/3950342‑3950588
                                                                                                                          |                                                                                                                            
gATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCt                                                                                                             >  1:163410/1‑139 (MQ=255)
 aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCtt                                                                                                            >  2:505467/1‑139 (MQ=255)
        tGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACa                                                                                                     <  1:433719/139‑1 (MQ=255)
         ggTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACAt                                                                                                    >  1:474874/1‑139 (MQ=255)
          gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACa                                                                                                     >  1:566320/1‑137 (MQ=255)
          gTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACa                                                                                                     <  2:566320/137‑1 (MQ=255)
                    gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGc                                                                                         >  2:286124/1‑139 (MQ=255)
                     cGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                                                        <  1:505467/139‑1 (MQ=255)
                                            cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                                                        <  1:107967/116‑1 (MQ=255)
                                            cGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                                                        >  2:107967/1‑116 (MQ=255)
                                             gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCtt                                                                                                            >  1:237906/1‑95 (MQ=255)
                                             gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCtt                                                                                                            <  2:237906/95‑1 (MQ=255)
                                                                   ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg                                          <  1:309414/139‑1 (MQ=255)
                                                                                    aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAAt                         >  1:432915/1‑139 (MQ=255)
                                                                                    aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAAt                         >  1:41640/1‑139 (MQ=255)
                                                                                         ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGgcgc                    <  2:41640/139‑1 (MQ=255)
                                                                                           aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCgaggag                                                                                          <  1:222140/67‑1 (MQ=255)
                                                                                           aaCGAACTAAGACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCgaggag                                                                                          >  2:222140/1‑67 (MQ=255)
                                                                                                     gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg                                          <  1:138535/105‑1 (MQ=255)
                                                                                                     gACCCCCGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACg                                          >  2:138535/1‑105 (MQ=255)
                                                                                                           cGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                      >  2:514257/1‑55 (MQ=255)
                                                                                                           cGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                                                      <  1:514257/55‑1 (MQ=255)
                                                                                                           cGCACCGAAAGGTCCGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGc  <  2:474874/139‑1 (MQ=255)
                                                                                                                          |                                                                                                                            
GATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAACTAACTATGAATGGCGCACAGTGGGTGGTACATGC  >  NC_000913/3950342‑3950588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: