Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 723,528 (T)8→7 coding (887/2685 nt) kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913723,5210T.100.0% 46.6 / NA 12coding (894/2685 nt)kdpDfused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/8);  total (4/8)

TCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAC  >  NC_000913/723397‑723616
                                                                                                                            |                                                                                               
tCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAg                                                                                  <  2:486254/139‑1 (MQ=255)
              tGTTCACGGACATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTgcagggcagg                                                                    <  2:94408/139‑1 (MQ=255)
                ttCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAgggg                                                                  <  1:106750/139‑1 (MQ=255)
                        cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGtttttttccg                                                                                        >  1:471352/1‑107 (MQ=255)
                        cATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGtttttttccg                                                                                        <  2:471352/110‑4 (MQ=255)
                                 cTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACcgcg                                                 >  1:78800/1‑139 (MQ=255)
                                                   cTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGtt                                                                <  1:137073/106‑1 (MQ=255)
                                                   cTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGtt                                                                >  2:137073/1‑106 (MQ=255)
                                                                                cccAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAc  <  1:503237/139‑1 (MQ=255)
                                                                                cccAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAc  <  2:78800/139‑1 (MQ=255)
                                                                                           cgcCAGACGTAAGGCGCTGAGAATTGCCCGACGtttttttccg                                                                                        <  1:182385/43‑4 (MQ=255)
                                                                                           cgcCAGACGTAAGGCGCTGAGAATTGCCCGACGtttttttccg                                                                                        >  2:182385/1‑40 (MQ=255)
                                                                                           cgcCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTc                                                              <  2:411484/68‑1 (MQ=255)
                                                                                           cgcCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGAAAGCGGTGCAGGGCAGGGGTTTc                                                              >  1:411484/1‑68 (MQ=255)
                                                                                               aGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACg                                        <  1:563811/86‑1 (MQ=255)
                                                                                               aGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACg                                        >  2:563811/1‑86 (MQ=255)
                                                                                                                            |                                                                                               
TCTTGCCGAGATTATGTTCACGGGCATAACGCACTACCGCTTTCTCTTCCGCTGGATCAGAAAGTGTTGCCGTCTCCGCGCCCAGTTCCTGCGCCAGACGTAAGGCGCTGAGAATTGCCCGACGTTTTTTTTCCGGTAAGCGGTGCAGGGCAGGGGTTTCAACATACACCGCGTGCCAGACGCTACCCAGCCGTGACGCCAGCCGCGCCGCTGCGCGGAC  >  NC_000913/723397‑723616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: