| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,606,215 | (G)8→10 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,606,207 | 1 | . | G | 100.0% | 34.2 / NA | 12 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (6/6); total (6/6) | |||||||||||
| * | NC_000913 | 4,606,207 | 2 | . | G | 83.3% | 28.5 / 1.1 | 12 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (1/1); new base G (5/5); total (6/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTT > NC_000913/4606090‑4606314 || gggggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAAccccc > 2:65129/1‑139 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT‑ACTTTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggg > 1:197853/1‑121 (MQ=255) gggACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAACTTCGCACAGTCATCTTAAT‑TTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑gggg < 2:197853/121‑1 (MQ=255) acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCt > 2:70795/1‑139 (MQ=255) tACCAATTCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCAAGCGCGTCTACCAATTCCGCCAcc > 1:356094/1‑139 (MQ=255) aTTCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAG‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 2:423986/139‑1 (MQ=255) ttCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:70795/139‑1 (MQ=255) ccACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc > 1:60131/1‑124 (MQ=255) ccACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc < 2:60131/124‑1 (MQ=255) cTTCGCACAGTCATGTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTTAATCCCCACGTCCGTAAGAACACTTACACCTGAATCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg < 2:356094/139‑1 (MQ=21) tCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc > 2:497792/1‑120 (MQ=255) tCATCTT‑AC‑TTTTTTTGATATCGCCTCCTTTGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc < 1:497792/120‑1 (MQ=18) tttGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt < 1:164856/119‑1 (MQ=255) tttGGTGCGAGGGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt > 2:164856/1‑119 (MQ=255) || GGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTT‑AC‑TTTTTTTGATATCGCCTCGTTTGGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTT > NC_000913/4606090‑4606314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |