Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	989,708	T→C	T217A (ACA→GCA)	pncB ←	nicotinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	989,708	0	T	C	100.0%	130.3 / NA	35	T217A (ACA→GCA)	pncB	nicotinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/19);  total (16/19)

ATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCA  >  NC_000913/989572‑989842                                                                                                                                         |                                                                                                                                       aTAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTc                                                                                                                                      >  2:518577/1‑139 (MQ=255)   aGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTccc                                                                                                                                    <  2:342983/139‑1 (MQ=255)   aGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTccc                                                                                                                                    >  2:24103/1‑139 (MQ=255)        tGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCgg                                                                                                                               >  2:519843/1‑139 (MQ=255)          cAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCg                                                                                                                             <  2:567470/139‑1 (MQ=255)             tCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGa                                                                                                                          >  2:274000/1‑139 (MQ=255)               ggTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAgg                                                                                                                        >  1:274245/1‑139 (MQ=255)                                      cGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACgcgc                                                                                                          <  1:69793/130‑1 (MQ=255)                                      cGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACgcgc                                                                                                          >  2:69793/1‑130 (MQ=255)                                               ctTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGt                                                                                        <  2:274245/139‑1 (MQ=255)                                                       cagGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACaa                                                                                <  1:24103/139‑1 (MQ=255)                                                          gcagcaAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCAt                                                                                                                                  >  1:20414/1‑86 (MQ=255)                                                          gcagcaAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCAt                                                                                                                                  <  2:20414/86‑1 (MQ=255)                                                                      gCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGAAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGtt                                                                 >  1:152224/1‑139 (MQ=255)                                                                         cGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCa                                                              <  1:51180/139‑1 (MQ=255)                                                                         cGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCa                                                              <  1:518577/139‑1 (MQ=255)                                                                                    ggCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCg                                                                                                                >  1:231656/1‑78 (MQ=255)                                                                                    ggCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCg                                                                                                                <  2:231656/78‑1 (MQ=255)                                                                                       tAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGAAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATg                                                <  2:152224/139‑1 (MQ=255)                                                                                         gATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGt                                              <  1:208183/139‑1 (MQ=255)                                                                                                    aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCca                                                                                   <  1:196335/91‑1 (MQ=255)                                                                                                    aTTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCca                                                                                   >  2:196335/1‑91 (MQ=255)                                                                                                                 ccTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCcaca                                                                                 <  1:395331/80‑1 (MQ=255)                                                                                                                 ccTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCcaca                                                                                 >  2:395331/1‑80 (MQ=255)                                                                                                                 ccTGGAACCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACg                      >  1:490530/1‑139 (MQ=255)                                                                                                                      aaCCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGt                                                                                               <  2:481183/61‑1 (MQ=255)                                                                                                                      aaCCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGt                                                                                               >  1:481183/1‑61 (MQ=255)                                                                                                                      aaCCATTCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGt                                                                                               >  1:379611/1‑61 (MQ=255)                                                                                                                      aaCCATTCGTGTGCCTGTACTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGt                                                                                               <  2:379611/61‑1 (MQ=255)                                                                                                                           ttCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAATACGGCGACGGGTg            >  2:423029/1‑139 (MQ=255)                                                                                                                            tCGTGTGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAATACGGCGACGGGTGc           <  1:423029/139‑1 (MQ=255)                                                                                                                              gtgtGCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCa         <  1:299872/139‑1 (MQ=255)                                                                                                                                 tgCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCa                                                              >  2:460157/1‑83 (MQ=255)                                                                                                                                 tgCCTGTGCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCa                                                              <  1:460157/83‑1 (MQ=255)                                                                                                                                     tgtgCTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCa  <  1:274000/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       ATAGTGATGCAGTCGGTTAATGCAATGCCAAGTTGGTCGGGATACTCTTCCAGCCAGGCAGCAAGTGCAGCTCGCTGGCTGTTGGCTAGATCCGGGCTGATTTGCTGATGTGCCTGGAACCATTCGTGTGCCTGTGTTCCCATCGGCGTGAGGGAAAGCCGACGCGCCAGATCGTAGTTGCTGGTGCCCACAAACCAGGATTCCTGTTGCAGACGCTTAACGATGGTTTCTTGTACTTCGCGAGAAAAACGGCGACGGGTGCCAAAATCCA  >  NC_000913/989572‑989842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑