Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,121,680	C→T	D310N (GAC→AAC)	pyrC ←	dihydro‑orotase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,121,680	0	C	T	100.0%	69.1 / NA	21	D310N (GAC→AAC)	pyrC	dihydro‑orotase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

AACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCC  >  NC_000913/1121570‑1121807                                                                                                               |                                                                                                                                aaCGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCggg                                                                                                     <  2:165717/139‑1 (MQ=255)   cGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                   <  2:118659/139‑1 (MQ=255)                     ccccGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGc                                                                                 >  1:86677/1‑139 (MQ=255)                         ggCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                   <  1:201161/117‑1 (MQ=255)                         ggCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                   >  2:201161/1‑117 (MQ=255)                                    acACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCaa                                                                  >  2:335129/2‑139 (MQ=255)                                      aCCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAg                                                                <  1:577037/139‑1 (MQ=255)                                             tgtCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGcattcat                                                         >  2:167905/1‑139 (MQ=255)                                                    agtcagtGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCa                                                  >  2:517063/1‑139 (MQ=255)                                                          tGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACg                                            >  2:468934/1‑139 (MQ=255)                                                               tGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCa                                                                                                                      <  1:491154/60‑1 (MQ=255)                                                               tGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCa                                                                                                                      >  2:491154/1‑60 (MQ=255)                                                                        cAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGcc                              <  1:517063/139‑1 (MQ=255)                                                                                 gCTCTTCACGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGt                     <  1:468934/139‑1 (MQ=255)                                                                                        aCGTACCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCg              <  1:335129/139‑1 (MQ=255)                                                                                            aCCAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGa          >  1:433206/1‑139 (MQ=255)                                                                                             ccAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                   <  2:88815/49‑1 (MQ=255)                                                                                             ccAGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGcc                                                                                                   >  1:88815/1‑49 (MQ=255)                                                                                               aGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaa                                                                                         >  2:487447/1‑57 (MQ=255)                                                                                               aGTTCGATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTacagaa                                                                                         <  1:487447/57‑1 (MQ=255)                                                                                                    gATGAATGTGTTGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGccc  >  1:128029/1‑139 (MQ=255)                                                                                                               |                                                                                                                                AACGGACCAGCGTACCGTTTCCCCGGCGAGGAATGGCACCAGCGTGTCATCAGTCAGTGCGATGCTTTCAGCAACCTGTTGCTCTTCACGTACCAGTTCGATGAATGTGTCGTTGACCGGCAACCCATAGAACTGCGGGCCGTTTACAGAACAGAATGCTTCAAAGTGCTGCAAAGCATTCATTTCTTCAAAGACGGTAGCGTAACTGCCCAGCGCGGTTGGGGCGTTGAAGCAGCCC  >  NC_000913/1121570‑1121807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑