Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,372,620 A→G T238A (ACT→GCT)  ycjN → putative ABC sugar transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,372,6200AG100.0% 77.1 / NA 21T238A (ACT→GCT) ycjNputative ABC sugar transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/11);  total (10/11)

GCAGAAAGCGTGTTGACGGAACAATCCTTCTCCCAGTTTGCGTTATCCAACCAGGCTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTACTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAA  >  NC_000913/1372485‑1372750
                                                                                                                                       |                                                                                                                                  
gcagAAAGCGTGTTGACGGAACAATCCTTCTCCCAGTTTGCGTTATCCAACCAGGCTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTg                                                                                                                                 >  1:568141/1‑139 (MQ=255)
                         ccTTCTCCCAGTTTGCGTTATCCAACCAGGCTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGa                                                                                                        <  2:120779/139‑1 (MQ=255)
                                                  ccAGGCTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTaa                                                                                                           <  1:251198/111‑1 (MQ=255)
                                                  ccAGGCTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTaa                                                                                                           >  2:251198/1‑111 (MQ=255)
                                                       cTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATg                                                                                                 <  1:249791/116‑1 (MQ=255)
                                                       cTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATg                                                                                                 >  2:249791/1‑116 (MQ=255)
                                                                ttAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAaca                                                                                                                           >  1:217810/1‑81 (MQ=255)
                                                                ttAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAaca                                                                                                                           <  2:217810/81‑1 (MQ=255)
                                                                        gAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATgg                                                         >  2:479052/1‑139 (MQ=255)
                                                                             cAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCGCCGCGCCGATGGCGAtt                                                    <  2:363345/139‑1 (MQ=255)
                                                                                  tCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTc                                               <  2:568141/139‑1 (MQ=255)
                                                                                  tCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTc                                               <  1:479052/139‑1 (MQ=255)
                                                                                              cacCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGcc                                                                                                                               <  1:428878/47‑1 (MQ=255)
                                                                                              cacCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTGCTGcc                                                                                                                               >  2:428878/1‑47 (MQ=255)
                                                                                                   gagaTGATGCAGGCACTGACCTATTACCGCGACCTtgctgc                                                                                                                                >  2:335169/1‑41 (MQ=255)
                                                                                                   gagaTGATGCAGGCACTGACCTATTACCGCGACCTtgctgc                                                                                                                                <  1:335169/41‑1 (MQ=255)
                                                                                                    agaTGATGCAGGCACTGACCTATTACCGCGACCTTGCTGcc                                                                                                                               <  2:321715/41‑1 (MQ=255)
                                                                                                    agaTGATGCAGGCACTGACCTATTACCGCGACCTTGCTGcc                                                                                                                               >  1:321715/1‑41 (MQ=255)
                                                                                                      atgatgCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCt                            <  2:130068/138‑1 (MQ=255)
                                                                                                      atgatgCAGGCACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCt                            >  1:130068/1‑138 (MQ=255)
                                                                                                                cACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATg                                                          >  1:454272/1‑98 (MQ=255)
                                                                                                                cACTGACCTATTACCGCGACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATg                                                          <  2:454272/98‑1 (MQ=255)
                                                                                                                               gcgACCTTGCTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGaaaaa  >  2:209986/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                  
GCAGAAAGCGTGTTGACGGAACAATCCTTCTCCCAGTTTGCGTTATCCAACCAGGCTAACGTCTTTAACGCCGAAGGCAAAATCACCCTTGATACACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTACTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAA  >  NC_000913/1372485‑1372750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: