Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,393,315	T→C	V30A (GTA→GCA)	mppA →	murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,393,315	0	T	C	100.0%	134.3 / NA	35	V30A (GTA→GCA)	mppA	murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/20);  total (15/20)

AAACAAATAATTACGCATCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTG  >  NC_000913/1393180‑1393433                                                                                                                                        |                                                                                                                       aaaCAAATAATTACGCATCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCGGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACt                                                                                                                     >  2:558796/1‑139 (MQ=255) aaaCAAATAATTACGCATCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACt                                                                                                                     >  2:419905/1‑139 (MQ=255)            tACGCATCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGc                                                                                                          >  2:23007/1‑139 (MQ=255)             aCGCATCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCa                                                                                                         >  2:466882/1‑139 (MQ=255)                      ttttAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGt                                                                                                <  2:364992/139‑1 (MQ=255)                          aaTGTCGGTTAGCGGGAAACTTATGAATCACTCTGTTTCAGTCATGTGTTGTGCTCTGTTGGTCCGCAGCATTTCTCCTGCGTTTGCTGCAGAACATCCGTGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgccc                                                                                            <  1:96816/139‑3 (MQ=255)                          aaTGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                            <  1:466882/139‑1 (MQ=255)                          aaTGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                            <  2:411433/139‑1 (MQ=255)                           aTGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGcc                                                                                           <  2:416611/139‑1 (MQ=255)                            tGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCGGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCca                                                                                          <  1:558796/139‑1 (MQ=255)                            tGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCca                                                                                          <  1:419905/139‑1 (MQ=255)                                ggTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCAGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCca                                                                                          <  1:382021/135‑1 (MQ=255)                                ggTTAGAGGGAAACTTATCAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCca                                                                                          >  2:382021/1‑135 (MQ=255)                                                           tGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCa                                                                                                         >  1:406530/1‑93 (MQ=255)                                                           tGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCa                                                                                                         <  2:406530/93‑1 (MQ=255)                                                                   tCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcg                                                                                             >  1:186486/1‑97 (MQ=255)                                                                   tCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcg                                                                                             <  2:186486/97‑1 (MQ=255)                                                                   tCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGc                                                   >  2:462665/1‑139 (MQ=255)                                                                       gtgtTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTg                                               >  1:134227/1‑139 (MQ=255)                                                                               cgcTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                            >  2:163779/1‑86 (MQ=255)                                                                               cgcTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTgcgc                                                                                            <  1:163779/86‑1 (MQ=255)                                                                                 cTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCag                                     <  1:462665/139‑1 (MQ=255)                                                                                              gcaTTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCAt                        >  1:533468/1‑139 (MQ=255)                                                                                                     tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                 <  1:23007/139‑1 (MQ=255)                                                                                                     tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                 <  2:134227/139‑1 (MQ=255)                                                                                                     tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                 <  2:134080/139‑1 (MQ=255)                                                                                                     tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                 <  1:55375/139‑1 (MQ=255)                                                                                                     tctTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGAt                 <  1:260213/139‑1 (MQ=255)                                                                                                         tCGTATGCTGCAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGTCTGCCAGAGATTCAGGTCATTCGCGATCTGt             >  1:140435/1‑139 (MQ=255)                                                                                                              tgctgcAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGaa        >  1:528830/1‑139 (MQ=255)                                                                                                                  gcAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTc    <  2:533468/139‑1 (MQ=255)                                                                                                                   cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGt                           >  1:134716/1‑115 (MQ=255)                                                                                                                   cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGt                           <  2:134716/115‑1 (MQ=255)                                                                                                                   cAGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGACCTGTTTGAAGGTCt   >  2:141786/1‑139 (MQ=255)                                                                                                                    aGAAGTTCCGAGCGGCACAGCACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTg  <  2:81782/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                       AAACAAATAATTACGCATCAATTTTAATGTCGGTTAGAGGGAAACTTATGAAGCACTCTGTTTCAGTCACGTGTTGTGCGCTGTTGGTCAGCAGCATTTCTCTTTCGTATGCTGCAGAAGTTCCGAGCGGCACAGTACTGGCAGAGAAGCAGGAGCTGGTGCGCCACATTAAAGATGAGCCTGCGTCGCTGGATCCCGCTAAAGCCGTGGGCCTGCCAGAGATTCAGGTCATTCGCGATCTGTTTGAAGGTCTG  >  NC_000913/1393180‑1393433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑