| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 261,052 | A→G | D222G (GAC→GGC) | proB → | gamma‑glutamate kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 261,052 | 0 | A | G | 95.7% | 80.0 / ‑5.6 | 23 | D222G (GAC→GGC) | proB | gamma‑glutamate kinase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/10); minor base C (0/1); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGCAGAACTGATTAAAGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAA > NC_000913/260937‑261178 | caggcagAACTGATTAAAGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCg < 1:103434/139‑1 (MQ=255) aGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTg > 2:581026/1‑113 (MQ=255) aGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTg < 1:581026/113‑1 (MQ=255) aGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGac > 1:299815/1‑124 (MQ=255) aGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGac < 2:299815/124‑1 (MQ=255) ttACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGgcagc > 2:496330/1‑139 (MQ=255) atgaCGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGCCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGt < 2:437138/139‑1 (MQ=255) gCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTg < 1:496330/139‑1 (MQ=255) cTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATc > 1:200175/1‑96 (MQ=255) cTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATc < 2:200175/96‑1 (MQ=255) gcgcgcGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGgtgat > 1:285290/1‑139 (MQ=255) cgcgcgATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATc > 1:252275/1‑93 (MQ=255) cgcgcgATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATc < 2:252275/93‑1 (MQ=255) cgcgcgATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGgcag < 1:52539/116‑1 (MQ=255) cgcgcgATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGgcag > 2:52539/1‑116 (MQ=255) cGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTAt > 2:356475/1‑72 (MQ=255) cGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTAt < 1:356475/72‑1 (MQ=255) aTGAGTACCAAATTGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGa > 1:262716/1‑139 (MQ=255) tGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGtgatgtgatg < 2:214741/86‑1 (MQ=255) tGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGtgatgtgatg > 1:214741/1‑86 (MQ=255) tGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTcc < 2:145259/114‑1 (MQ=255) tGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTcc > 1:145259/1‑114 (MQ=255) tGCAGGCCGCTGGCGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGaaaa > 1:448375/1‑139 (MQ=255) | CAGGCAGAACTGATTAAAGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCATGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGCGTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAA > NC_000913/260937‑261178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |