Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,520,008 C→T L328L (CTG→TTG)  curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,520,0080CT100.0% 75.5 / NA 23L328L (CTG→TTG) curAcurcumin/dihydrocurcumin reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/12);  total (11/12)

CGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCA  >  NC_000913/1519878‑1520137
                                                                                                                                  |                                                                                                                                 
cGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAg                                                                                                                           >  1:215963/1‑139 (MQ=255)
cGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAg                                                                                                                           >  1:45119/1‑139 (MQ=255)
 gCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAGAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAgg                                                                                                                          >  2:466756/1‑139 (MQ=255)
                cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAGAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  1:466756/139‑1 (MQ=255)
                cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  2:45119/139‑1 (MQ=255)
                cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  2:117657/139‑1 (MQ=255)
                cACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  2:215963/139‑1 (MQ=255)
                 aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGACAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCaa                                                                                                          >  2:378786/1‑139 (MQ=255)
                 aCCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCaa                                                                                                          >  1:62981/1‑139 (MQ=255)
                   cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGa                                                                                                                             >  1:431949/1‑118 (MQ=255)
                   cGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGa                                                                                                                             <  2:431949/118‑1 (MQ=255)
                                     agGGAGATGGGTCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGACTGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCgg                                                                                      <  2:360550/139‑1 (MQ=255)
                                           aTGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGtgatg                                                                                >  2:41621/1‑139 (MQ=255)
                                                                                                cGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           >  2:542986/1‑59 (MQ=255)
                                                                                                cGGTTTAGAGAATGCGCCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  1:542986/59‑1 (MQ=255)
                                                                                                             gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           >  2:458368/1‑46 (MQ=255)
                                                                                                             gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCa                                                                                                           <  1:458368/46‑1 (MQ=255)
                                                                                                             gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAtt                                                                             <  2:157174/76‑1 (MQ=255)
                                                                                                             gcgcCACAGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGAtt                                                                             >  1:157174/1‑76 (MQ=255)
                                                                                                                 cacaGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGt                                                                                    <  1:167170/65‑1 (MQ=255)
                                                                                                                 cacaGACGTTTATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGt                                                                                    >  2:167170/1‑65 (MQ=255)
                                                                                                                         tttATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACca  <  1:378786/139‑1 (MQ=255)
                                                                                                                         tttATCGGCTTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACca  <  2:62981/139‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
CGCTCAGGATTATGGTCACCGCATCCATGAGTTTCAGAGGGAGATGGGGCAATGGGTGAAAGAGGATAAAATCCACTACCGCGAAGAAATTACTGACGGTTTAGAGAATGCGCCACAGACGTTTATCGGCCTGCTGAAGGGTAAAAACTTCGGCAAAGTGGTGATCCGCGTGGCGGGTGATGATTAAAGTTTCAACGGCGGCGTAAGCCGCCATAAAAATCCAGGCAAAAAGCTTATTTTCAGCTTTAATTAACTAACCA  >  NC_000913/1519878‑1520137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: