Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,688,142	G→A	A79V (GCC→GTC)	fumA ←	fumarate hydratase (fumarase A), aerobic Class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,688,142	0	G	A	100.0%	47.4 / NA	15	A79V (GCC→GTC)	fumA	fumarate hydratase (fumarase A), aerobic Class I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)

GCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTT  >  NC_000913/1688023‑1688274                                                                                                                        |                                                                                                                                     gCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAAt                                                                                                                   >  1:248842/1‑139 (MQ=255)  cccTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATg                                                                                                                  >  1:548859/1‑139 (MQ=255)                           gTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGc                                                                                                             >  1:96330/1‑119 (MQ=255)                           gTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGc                                                                                                             <  2:96330/119‑1 (MQ=255)                                ggTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgc                                                                                    <  2:548859/139‑1 (MQ=255)                                           aGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacga                                                                         <  1:542160/139‑1 (MQ=255)                                            ggTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAacgac                                                                        <  2:248842/139‑1 (MQ=255)                                                ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGcat                                                                    >  1:524659/1‑139 (MQ=255)                                                                        tCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTATTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACa                                            >  1:189734/1‑139 (MQ=255)                                                                                      gAATTGCAGCGCCACATATTTATCATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCg                              >  2:161442/1‑139 (MQ=255)                                                                                                             cATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGc                         >  1:367230/1‑121 (MQ=255)                                                                                                             cATTTTCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGc                         <  2:367230/121‑1 (MQ=255)                                                                                                                 ttCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCt   >  1:296550/1‑139 (MQ=255)                                                                                                                 ttCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCt   >  1:372295/1‑139 (MQ=255)                                                                                                                  tCGCTGACCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCtt  >  1:384771/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                     GCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAACGACGCATCATGAAACGCCTGGCGCGCCAACAGAGTTAACGCTTCGGGTGCGACTTTCAAAATCTCCTGCCCTT  >  NC_000913/1688023‑1688274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑