Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,491	G→A	G41D (GGC→GAC)	pfkB →	6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,491	0	G	A	100.0%	59.5 / NA	18	G41D (GGC→GAC)	pfkB	6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/7);  total (11/7)

TGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAG  >  NC_000913/1806389‑1806619                                                                                                       |                                                                                                                                 tGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAgg                                                                                              >  1:5631/1‑139 (MQ=255)  gACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGc                                                                                             >  1:368851/1‑139 (MQ=255)  gACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGc                                                                                             >  2:485406/1‑139 (MQ=255)   acacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCa                                                                                            >  1:482151/1‑139 (MQ=255)   acacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCa                                                                                            >  2:236607/1‑139 (MQ=255)    cacTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAg                                                                                           <  1:236607/139‑1 (MQ=255)                             aacaaTTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGTGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGt                                                                  >  2:175796/1‑139 (MQ=255)                             aacaaTTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGt                                                                  >  1:276759/1‑139 (MQ=255)                                  ttACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGgcgc                                                             <  2:482151/139‑1 (MQ=255)                                    aCCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGa                                                           <  2:368851/139‑1 (MQ=255)                                                                             aCCGGTGTTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTc                  >  1:559488/1‑139 (MQ=255)                                                                                 gtgtTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg              >  2:489677/1‑139 (MQ=255)                                                                                 gtgtTCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCg              >  2:397682/1‑139 (MQ=255)                                                                                    ttCGAACCCGGGGGCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCg           <  2:570106/139‑1 (MQ=255)                                                                                             gggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg  <  1:489677/139‑1 (MQ=255)                                                                                             gggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAg  <  2:5631/139‑1 (MQ=255)                                                                                              ggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt             <  2:252728/127‑1 (MQ=255)                                                                                              ggggCGGCGACATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGt             >  1:252728/1‑127 (MQ=255)                                                                                                       |                                                                                                                                 TGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTACCGCACCGGTGTTCGAACCCGGGGGCGGCGGCATCAACGTCGCCCGCGCCATTGCCCATCTTGGAGGCAGTGCCACAGCGATCTTCCCGGCGGGTGGCGCGACCGGCGAACACCTGGTTTCACTGTTGGCGGATGAAAATGTCCCCGTCGCTACTGTAG  >  NC_000913/1806389‑1806619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑