Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 265,137 C→T V24I (GTC→ATC)  yafX ← CP4‑6 prophage, uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,1370CT100.0% 62.3 / NA 19V24I (GTC→ATC) yafXCP4‑6 prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/9);  total (10/9)

CCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGACCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATA  >  NC_000913/265009‑265260
                                                                                                                                |                                                                                                                           
ccAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACg                                                                                                                   >  2:82521/1‑139 (MQ=255)
ccAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACg                                                                                                                   >  1:476136/1‑139 (MQ=255)
        caccGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTc                                                                                                           <  1:546435/139‑1 (MQ=255)
           cGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCaaa                                                                                                        >  1:141443/1‑139 (MQ=255)
              tGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTc                                                                                                     >  2:241395/1‑139 (MQ=255)
                       cacaGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGggttggtt                                                                                            >  2:160456/1‑139 (MQ=255)
                                 gTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCg                                                                                  <  2:476136/139‑1 (MQ=255)
                                  tccatccaGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCggg                                                                                                  <  2:567909/122‑1 (MQ=255)
                                  tccatccaGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCggg                                                                                                  >  1:567909/1‑122 (MQ=255)
                                            cGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGtgctg                                                                       <  2:141443/139‑1 (MQ=255)
                                                               gAGTAGCCAATGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGt                                                    <  1:342209/139‑1 (MQ=255)
                                                                   aGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGt                                                >  2:168532/1‑139 (MQ=255)
                                                                                                     aGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGggt                                                                                                 >  2:249182/1‑56 (MQ=255)
                                                                                                     aGAAATCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGggt                                                                                                 <  1:249182/56‑1 (MQ=255)
                                                                                                       aaaTCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCggg                                                                                                  <  1:311783/53‑1 (MQ=255)
                                                                                                       aaaTCGATACGCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCggg                                                                                                  >  2:311783/1‑53 (MQ=255)
                                                                                                             aTACGCTGTTCACCGGGGATCGGCGGGACGGTCAGTTTAAATTCGTGTTGGTTAGCGGATGAGAGGTCGTGCTGCGTCTGTGTTGTCCTGGGTCTGTCTCCGCCAATAAAGACGCCAGCGCCGATGGCTGGCGTATggg      <  1:159035/139‑1 (MQ=21)
                                                                                                                 gCTGTTCATCGGGGATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGata  <  1:362180/139‑1 (MQ=255)
                                                                                                                            gggATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGTGGGTGCGAGGTCGtg                                                                          >  2:18033/1‑56 (MQ=39)
                                                                                                                            gggATCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGTGGGTGCGAGGTCGtg                                                                          <  1:18033/56‑1 (MQ=39)
                                                                                                                                |                                                                                                                           
CCAGATACCACCGCTGTAGCCCTCACAGAAGCGGTCCATCCAGGCGAAGATATGCGGCTCCAGGAGTAGCCACTGCGGGATAGCGCCAAAGTACTGTGGCCAGAAATCGATACGCTGTTCATCGGGGACCGGCGTGACGGTCAGTTCAAATTCGGGTTGGTTAGCGGGTGCGAGGTCGTGCTGCGTCTGTGTTGTCATGGGTATGTCTCCGTCAATAAAAACGCCAGCGGCGATGGCTGGCGTATGGGGATA  >  NC_000913/265009‑265260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: