Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,000,873 C→T R51H (CGC→CAC)  fliZ ← RpoS antagonist, putative regulator of FliA activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,000,8730CT100.0% 72.7 / NA 22R51H (CGC→CAC) fliZRpoS antagonist, putative regulator of FliA activity
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/11);  total (11/11)

ATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGT  >  NC_000913/2000756‑2001001
                                                                                                                     |                                                                                                                                
ataataTCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCa                                                                                                             >  1:143357/1‑139 (MQ=255)
         aaaaaaTCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGc                                                                                                    >  1:43064/1‑139 (MQ=255)
              aTCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTcg                                                                                               <  1:399629/139‑1 (MQ=255)
                cACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTcgcg                                                                                             <  2:472840/139‑1 (MQ=255)
                      gCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGaa                                                                                           <  1:345353/135‑1 (MQ=255)
                      gCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTGGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGaa                                                                                           >  2:345353/1‑135 (MQ=255)
                                     aaTCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAg                                                                        <  1:443150/139‑1 (MQ=255)
                                           cGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACt                                                                                         >  1:284318/1‑116 (MQ=255)
                                           cGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACt                                                                                         <  2:284318/116‑1 (MQ=255)
                                                     gCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATgc                                                        >  2:404862/1‑139 (MQ=255)
                                                                cccATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGt                                             <  2:143357/139‑1 (MQ=255)
                                                                cccATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGt                                             >  2:24841/1‑139 (MQ=255)
                                                                     gATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCg                                                                                                                       <  2:498717/60‑1 (MQ=255)
                                                                     gATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCg                                                                                                                       >  1:498717/1‑60 (MQ=255)
                                                                     gATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGc                                        >  1:157820/1‑139 (MQ=255)
                                                                             ttCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTa                                <  1:24841/139‑1 (MQ=255)
                                                                               ccGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTaaa                              <  1:547291/139‑1 (MQ=255)
                                                                               ccGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTCGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTaaa                              <  1:404862/139‑1 (MQ=255)
                                                                                  tttGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTaa                                                                                  >  2:317898/1‑84 (MQ=255)
                                                                                  tttGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTaa                                                                                  <  1:317898/84‑1 (MQ=255)
                                                                                                           cGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGt  >  1:35898/1‑139 (MQ=255)
                                                                                                           cGTGTCCAGGTGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGt  >  2:518363/1‑139 (MQ=255)
                                                                                                                     |                                                                                                                                
ATAATATCGAAAAAATCACTCTGCGTTTTGCAATGTAAATCACCGCAAAATCGGCACAATGCCGCCCATGATTTTTGTTCCGTTTGCCAGCCATTTTCATCAAGCAGCGTGTCCAGGCGGGAAATCTCGATTTTATTCACTATTTTGCCGTCGCGAACTAAGGTAATGCGATCGAGTAATTTACGGCAATGCGCGCAATGGGTCTGGCTGTGTTTAAAGTCTTTAAGATAGCGGCTTAATGGCCGT  >  NC_000913/2000756‑2001001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: