Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,230,208	G→A	P60S (CCA→TCA)	dusC ←	tRNA‑dihydrouridine synthase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,230,208	0	G	A	100.0%	40.7 / NA	13	P60S (CCA→TCA)	dusC	tRNA‑dihydrouridine synthase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

TTTCGACGGGCAGCCGCAATTGAGATCCACGCCCCAGGAACCTAACTCCACCGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGGTGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTT  >  NC_000913/2230083‑2230321                                                                                                                              |                                                                                                                  tttCGACGGGCAGCCGCAATTGAGATCCACGCCCCAGGAACCTAACTCCACCGCATGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCg                                                                                                      >  2:251969/1‑139 (MQ=255)                            cACGCCCCAGGAACCTAACTCCACCGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGa                                                                           >  2:536059/1‑139 (MQ=255)                              cGCCCCAGGAACCTAACTCCACCGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTc                                                                                           <  2:386659/121‑1 (MQ=255)                              cGCCCCAGGAACCTAACTCCACCGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTCTTGTAGCTc                                                                                           >  1:386659/1‑121 (MQ=255)                                            aaCTCCACCGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGgca                                                           <  1:256776/139‑1 (MQ=255)                                                   ccGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGgcagc                                                         <  1:140802/134‑1 (MQ=255)                                                   ccGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGgcagc                                                         >  2:140802/1‑134 (MQ=255)                                                         gggCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATccac                                              >  1:557543/1‑139 (MQ=255)                                                         gggCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATccac                                              >  2:253107/1‑139 (MQ=255)                                                                         cagccaTTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCg                              >  2:1844/1‑139 (MQ=255)                                                                          agccaTTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCgg                             <  1:251969/139‑1 (MQ=255)                                                                                 gtgGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGca                      <  2:557543/139‑1 (MQ=255)                                                                                                     tGCACGCGCACCAGCGTACCAGATGATGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACtt  >  1:578035/1‑139 (MQ=255)                                                                                                                              |                                                                                                                  TTTCGACGGGCAGCCGCAATTGAGATCCACGCCCCAGGAACCTAACTCCACCGCACGGGCGGCGTTCTCTGCCAGCCATTGTGGGAACTGACCTAACAACTGCACGCGCACCAGCGTACCAGATGGTGTCCGGCTGGCGTTTTGTAGCTCAGGGCAAATGCGATGAAAGACTTTTACCGGCAGCAGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTT  >  NC_000913/2230083‑2230321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑