Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,607,273	C→T	F119F (TTC→TTT)	hyfF →	hydrogenase 4, membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,607,273	0	C	T	100.0%	103.3 / NA	30	F119F (TTC→TTT)	hyfF	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

GTCTGGGCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGAAGC  >  NC_000913/2607137‑2607407                                                                                                                                         |                                                                                                                                       gTCTGGGCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCa                                                                                                                                      <  1:89062/139‑1 (MQ=255)   cTGGGCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATc                                                                                                                                    >  1:523916/1‑139 (MQ=255)     gggCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTg                                                                                                                                  >  1:274784/1‑139 (MQ=255)      ggCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGt                                                                                                                                 <  2:523916/139‑1 (MQ=255)      ggCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCAACTGt                                                                                                                                 >  2:382418/1‑139 (MQ=255)                     gCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCAcc                                                                                                                      >  2:528637/1‑135 (MQ=255)                     gCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCAcc                                                                                                                      <  1:528637/135‑1 (MQ=255)                           cTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTgg                                                                                                            <  2:274784/139‑1 (MQ=255)                              ggTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGgttg                                                                                                         <  2:138078/139‑1 (MQ=255)                                        gCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCaa                                                                                               <  2:446756/139‑1 (MQ=255)                                                               aTTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGggcgg                                                                        >  1:78518/1‑139 (MQ=255)                                                               aTTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGAGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGggcag                                                                        >  1:180009/1‑137 (MQ=255)                                                                   gCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTAc                                                                                                     >  2:220293/1‑106 (MQ=255)                                                                   gCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTAc                                                                                                     <  1:220293/106‑1 (MQ=255)                                                                   gCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCa                                                                                                   >  1:270990/1‑108 (MQ=255)                                                                   gCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCa                                                                                                   <  2:270990/108‑1 (MQ=255)                                                                                 gAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAAcc                                                                                          <  2:22439/103‑1 (MQ=255)                                                                                 gAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAAcc                                                                                          >  1:22439/1‑103 (MQ=255)                                                                                       gCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCt                                                >  2:505104/1‑139 (MQ=255)                                                                                        cacaCGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTc                                               <  2:78518/139‑1 (MQ=255)                                                                                             ggCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATaa                                                                                            <  1:198257/89‑1 (MQ=255)                                                                                             ggCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATaa                                                                                            >  2:198257/1‑89 (MQ=255)                                                                                                       cACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCa                                                                                                                        <  1:525755/51‑1 (MQ=255)                                                                                                       cACCCGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCa                                                                                                                        >  2:525755/1‑51 (MQ=255)                                                                                                           cGTTACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCAtt                            <  1:505104/139‑1 (MQ=255)                                                                                                             ttACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCAtgctgc                                                                                                               >  2:164310/1‑54 (MQ=255)                                                                                                             ttACGCTGTGCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCAtgctgc                                                                                                               <  1:164310/54‑1 (MQ=255)                                                                                                                    gtgCGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAg                   >  2:93788/1‑139 (MQ=255)                                                                                                                       cGATTACTACGGTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGt                >  1:393374/1‑139 (MQ=255)                                                                                                                                  gTTTCTTTCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGa     <  2:393374/139‑1 (MQ=255)                                                                                                                                    ttctttCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGAAg   >  1:219299/1‑139 (MQ=255)                                                                                                                                     tctttCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGAAGc  <  1:393998/139‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       GTCTGGGCGGTTTGTTCCTCGCCATTCTTGGTGTGATTGGCTTTCTCACCGGTATTTACTCGATTGGCTACATGCGTCATGAAGTGGCACACGGCGAGCTTTCACCCGTTACGCTGTGCGATTACTACGGTTTCTTCCATCTGTTTTTGTTCACCATGCTGCTGGTTGTTACCAGCAATAACCTGATTGTGATGTGGGCGGCGATCGAAGCCACCACCTTAAGCTCGGCGTTTCTGGTAGGCATTTACGGTCAGCGTTCATCGCTGGAAGC  >  NC_000913/2607137‑2607407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑