| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,619,314 | T→C | T283A (ACG→GCG) | uraA ← | uracil permease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,619,314 | 0 | T | C | 91.7% | 69.9 / ‑1.5 | 24 | T283A (ACG→GCG) | uraA | uracil permease |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (10/12); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
CGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATCTTTTTTGACGATATTAGCCGTTACTACCAGGTG > NC_000913/2619188‑2619428 | cGGCAGCCAGTTTACCGACGCAGGAAAGCAGGACAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAaagaa > 2:363241/1‑139 (MQ=255) aaTCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa < 1:363241/139‑1 (MQ=255) aaTCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa < 1:222720/139‑1 (MQ=255) ccgccccgccGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg < 2:533471/139‑1 (MQ=255) cccccccgccGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg < 2:505966/136‑1 (MQ=255) tGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa < 2:56182/110‑1 (MQ=255) tGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa > 1:56182/1‑110 (MQ=255) tGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCACAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATc > 1:181990/1‑139 (MQ=255) gTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATCt > 1:142232/1‑139 (MQ=255) gggTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATCTTTTTTGAc > 2:416698/1‑139 (MQ=255) ggTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg > 1:17030/1‑105 (MQ=255) ggTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg < 2:17030/105‑1 (MQ=255) aTCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa < 2:451314/96‑1 (MQ=255) aTCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa > 1:451314/1‑96 (MQ=255) aTCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg < 1:509777/101‑1 (MQ=255) aTCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg > 2:509777/1‑101 (MQ=255) gTTTTCTCCGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATCTTTTTTGACGATATTAGCCGTTACTACCAgg < 2:142232/139‑1 (MQ=255) tttCTCCGTAAGTAGTATTTGGCACAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATCTTTTTTGACGATATTAGCCGTTACTACCAGGTg < 2:181990/139‑1 (MQ=255) cGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa < 1:571639/71‑1 (MQ=255) cGTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGaa > 2:571639/1‑71 (MQ=255) gTACGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg > 2:394760/1‑75 (MQ=255) gTAAGTAGTATTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg < 1:394760/75‑1 (MQ=255) taTTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg > 2:250533/1‑67 (MQ=255) taTTTGGCGCAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTg < 1:250533/67‑1 (MQ=255) | CGGCAGCCAGTTTACCGACGCAGGAAAGCAGGATAGCGAAAATCGCCGCCCCGCCGATAACCCAGGTACTGTAAACACGGGTGATCGCCATCACGCCAATGTTTTCTCCGTAAGTAGTATTTGGCGTAGAGCCAAAGAAGCCGGAAATCACGGTCGACAAGCCATTAGCAAACATCGAACGGTGCAGACCTGGATCGCGCAGCAGATCTTTTTTGACGATATTAGCCGTTACTACCAGGTG > NC_000913/2619188‑2619428 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |