Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,852,122 G→A G87S (GGC→AGC)  hypC → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,852,1220GA100.0% 87.0 / NA 26G87S (GGC→AGC) hypChydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

CGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGA  >  NC_000913/2851990‑2852244
                                                                                                                                    |                                                                                                                          
cgcgTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAgg                                                                                                                      >  2:418518/1‑139 (MQ=255)
                               gCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcgc                                                                                       >  2:94688/1‑139 (MQ=255)
                                                  taatGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCTCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGc                                                                    <  1:418518/139‑1 (MQ=255)
                                                         gCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAAt                                                             >  2:48716/1‑139 (MQ=255)
                                                              aGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGATTATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGc                                                        <  1:94688/139‑1 (MQ=255)
                                                                    cgACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcgcgcg                                                                                    >  1:345867/1‑105 (MQ=255)
                                                                    cgACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcgcgcg                                                                                    <  2:345867/105‑1 (MQ=255)
                                                                        acTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGa                                              >  2:152316/1‑139 (MQ=255)
                                                                          tctcGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAAc                                            <  1:245264/139‑1 (MQ=255)
                                                                             cGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTg                                         >  1:316762/1‑139 (MQ=255)
                                                                                     tACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACAt                                 <  1:152316/139‑1 (MQ=255)
                                                                                      aCAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGc                                                        <  2:64389/115‑1 (MQ=255)
                                                                                      aCAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGc                                                        >  1:64389/1‑115 (MQ=255)
                                                                                               gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCg                                                                                                          <  1:386248/56‑1 (MQ=255)
                                                                                               gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCg                                                                                                          >  2:386248/1‑56 (MQ=255)
                                                                                               gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTgcg                                                 <  2:527626/113‑1 (MQ=255)
                                                                                               gTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTgcg                                                 >  1:527626/1‑113 (MQ=255)
                                                                                                tttGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcg                                                                                        >  1:166998/1‑73 (MQ=255)
                                                                                                tttGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATcg                                                                                        <  2:166998/73‑1 (MQ=255)
                                                                                                          aGCCGGATGTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGc            >  1:491912/1‑139 (MQ=255)
                                                                                                                  gTCGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg    <  2:491912/139‑1 (MQ=255)
                                                                                                                    cGGCGCGCTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGa  >  1:4973/1‑139 (MQ=255)
                                                                                                                          gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg    <  2:446726/131‑1 (MQ=255)
                                                                                                                          gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg    >  1:511636/1‑131 (MQ=255)
                                                                                                                          gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg    <  2:511636/131‑1 (MQ=255)
                                                                                                                          gcTGTTGTATAGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCg    >  1:446726/1‑131 (MQ=255)
                                                                                                                                    |                                                                                                                          
CGCGTGGGCCAGTGGGTACTGGTACACGTTGGCTTTGCCATGAGCGTAATTAATGAAGCCGAAGCACGCGACACTCTCGACGCCTTACAAAACATGTTTGACGTTGAGCCGGATGTCGGCGCGCTGTTGTATGGCGAGGAAAAATAATGCGTTTTGTTGATGAATATCGCGCGCCGGAACAGGTGATGCAGTTAATTGAGCATCTGCGCGAACGTGCTTCACATCTCTCTTACACCGCCGAACGCCCTCTGCGGA  >  NC_000913/2851990‑2852244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: