Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,139,583	G→A	R286H (CGT→CAT)	yghU →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,139,583	0	G	A	100.0%	83.3 / NA	25	R286H (CGT→CAT)	yghU	putative S‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/15);  total (10/15)

CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATC  >  NC_000913/3139447‑3139717                                                                                                                                           |                                                                                                                                       cGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATc                                                                                                                                      <  2:70303/139‑1 (MQ=255)      ggCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAgggg                                                                                                                                 >  2:169651/1‑139 (MQ=255)       gCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGt                                                                                                                                <  1:526232/139‑1 (MQ=255)         gAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTaa                                                                                                                              <  1:537788/139‑1 (MQ=255)                     cGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtc                                                                                                                  >  1:390838/1‑139 (MQ=255)                       aaCGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTtcgt                                                                                                                <  1:168008/139‑1 (MQ=255)                              ggcggTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgcagca                                                                                                         >  1:482487/1‑139 (MQ=255)                                cggTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAg                                                                                                       <  1:362034/139‑1 (MQ=255)                                     aaaCGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATc                                                                                                  <  2:390838/139‑1 (MQ=255)                                                         ccGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCa                                                                              <  1:169651/139‑1 (MQ=255)                                                             aCCAACGGACCGCTGAATGAGCAGTGTTGCATGAGCGCCATGACGCCGGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCAcg                                                                            >  2:83836/1‑139 (MQ=255)                                                                 aCGGACCGCTCAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCAt                                                                      >  2:486393/1‑139 (MQ=255)                                                                     aCCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                  <  2:152325/139‑1 (MQ=255)                                                                       cGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGgtg                                                                                                                     >  2:226130/1‑86 (MQ=255)                                                                       cGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGgtg                                                                                                                     <  1:226130/86‑1 (MQ=255)                                                                       cGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCa                                                                <  2:190272/139‑1 (MQ=255)                                                                                 gCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGcaca                                                      <  1:424549/139‑1 (MQ=255)                                                                                    gtgTTGCATGAGCGCCATGACGCCGGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACaa                                                     <  1:83836/137‑1 (MQ=255)                                                                                          cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAg                                                                                                       <  2:174037/83‑1 (MQ=255)                                                                                          cATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAg                                                                                                       >  1:174037/1‑83 (MQ=255)                                                                                                                aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgca                                                                                                            >  1:533189/1‑56 (MQ=255)                                                                                                                aTTTCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCgca                                                                                                            <  2:533189/56‑1 (MQ=255)                                                                                                                  ttCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAg                                                                                               >  1:432125/1‑67 (MQ=255)                                                                                                                  ttCGAGACGAATACGGAAGATAAGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAg                                                                                               <  2:432125/67‑1 (MQ=255)                                                                                                                                       aaGCATCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAatcatc  >  1:328881/1‑139 (MQ=255)                                                                                                                                           |                                                                                                                                       CGCTGGGCGAAAGAAGTAGGCGAACGTCCGGCGGTGAAACGTGGGCGTATTGTTAACCGCACCAACGGACCGCTGAATGAGCA‑‑GTTGCATGAGCGCCATGACGCCAGTGATTTCGAGACGAATACGGAAGATAAGCGTCAGGGGTAAGGGTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACAATGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATC  >  NC_000913/3139447‑3139717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑