Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,457,094	A→G	I240V (ATC→GTC)	gspD →	general secretory pathway component, cryptic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,457,094	0	A	G	100.0%	66.4 / NA	18	I240V (ATC→GTC)	gspD	general secretory pathway component, cryptic
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/9);  total (9/9)

GAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGG  >  NC_000913/3456968‑3457183                                                                                                                               |                                                                                          gAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAGTTAATCAGCAAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGAc                                                                               >  1:357417/1‑139 (MQ=255)            aGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCa                                                                    <  1:125187/139‑1 (MQ=255)                                   ttAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACt                                             >  1:276912/1‑139 (MQ=255)                                                      aaGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGaa                          <  1:305377/139‑1 (MQ=255)                                                          cACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAagaga                      <  1:297571/139‑1 (MQ=255)                                                                   aGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGAACCGGAAAAAGCACGCCAGCGCATCa                                                       >  2:396539/1‑97 (MQ=255)                                                                   aGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGAACCGGAAAAAGCACGCCAGCGCATCa                                                       <  1:396539/97‑1 (MQ=255)                                                                         aTGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATa       >  1:122825/1‑139 (MQ=255)                                                                           gCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATAcc     <  2:122825/139‑1 (MQ=255)                                                                              aGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCgg  >  1:432064/1‑139 (MQ=255)                                                                                    cctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCa                                                          >  1:68465/1‑77 (MQ=255)                                                                                    cctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCa                                                          <  2:68465/77‑1 (MQ=255)                                                                                    cctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACtt                                                    >  2:176067/1‑83 (MQ=255)                                                                                    cctcTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACtt                                                    <  1:176067/83‑1 (MQ=255)                                                                                        tcCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGa                                                                                <  1:529811/51‑1 (MQ=255)                                                                                        tcCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTGTCATCAGTGGa                                                                                >  2:529811/1‑51 (MQ=255)                                                                                                               gCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                         >  1:181887/1‑51 (MQ=255)                                                                                                               gCGAACCAACTCTCTTGTCATCAGTGGACCGGAAAAAGCACGCCAGCGCAt                                                         <  2:181887/51‑1 (MQ=255)                                                                                                                               |                                                                                          GAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCATCAGTGGACCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGG  >  NC_000913/3456968‑3457183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑