Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,530,091 G→A T106T (ACG→ACA hslO → heat shock protein Hsp33

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,0910GA100.0% 96.9 / NA 29T106T (ACG→ACAhslOheat shock protein Hsp33
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/16);  total (13/16)

ATATCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGA  >  NC_000913/3529964‑3530198
                                                                                                                               |                                                                                                           
atatCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCaa                                                                                                  <  2:206677/139‑1 (MQ=255)
         tACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCgg                                                                                                     >  1:237455/1‑127 (MQ=255)
         tACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCgg                                                                                                     <  2:237455/127‑1 (MQ=255)
              cTGCGGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGa                                                                                    >  2:426455/1‑139 (MQ=255)
              cTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGa                                                                                    >  2:490900/1‑139 (MQ=255)
               tGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                   <  1:136479/139‑1 (MQ=255)
               tGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                   <  1:329994/139‑1 (MQ=255)
                                        ggCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGaa                                                          <  2:300156/139‑1 (MQ=255)
                                                       tAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCa                                                                              <  2:445435/104‑1 (MQ=255)
                                                       tAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCa                                                                              >  1:445435/1‑104 (MQ=255)
                                                        aaCAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACg                                                                                          >  1:354941/1‑91 (MQ=255)
                                                        aaCAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACg                                                                                          <  2:354941/91‑1 (MQ=255)
                                                              aaCCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTc                                    <  1:426455/139‑1 (MQ=255)
                                                                    cagATGCGCGGTGTGGCGCGCGTGCAGGGCTAAATTCCAGAAAATGCCGACCTGAAAACAGTGGTCGGCAATGGTTACGTGGTGAt                                                                                   <  1:538987/86‑1 (MQ=255)
                                                                    cagATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTAGGCAATGGTTACGTGGTGAt                                                                                   >  2:538987/1‑86 (MQ=255)
                                                                     agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                   <  1:114921/85‑1 (MQ=255)
                                                                     agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGAt                                                                                   >  2:114921/1‑85 (MQ=255)
                                                                     agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg                                                                  >  2:27421/1‑102 (MQ=255)
                                                                     agATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg                                                                  <  1:27421/102‑1 (MQ=255)
                                                                       aTGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTg                           >  1:329141/1‑139 (MQ=255)
                                                                            cgGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg                                                                  <  1:443896/95‑1 (MQ=255)
                                                                            cgGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCg                                                                  >  2:443896/1‑95 (MQ=255)
                                                                                         gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTggcggc              <  2:335212/134‑1 (MQ=255)
                                                                                         gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTggcggc              >  1:335212/1‑134 (MQ=255)
                                                                                         gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGgcctgcc         <  2:329141/139‑1 (MQ=255)
                                                                                         gTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGAGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGgcctgcc         >  1:375002/1‑139 (MQ=255)
                                                                                               ggCGAAATTCCAGAAAATGCCGACCTGAAAACACTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGa  <  1:490900/139‑1 (MQ=255)
                                                                                                 cGAAATTCCAGAAAATGCCGACCTGAAAACACTGGt                                                                                                        >  1:434917/1‑36 (MQ=255)
                                                                                                 cGAAATTCCAGAAAATGCCGACCTGAAAACACTGGt                                                                                                        <  2:434917/36‑1 (MQ=255)
                                                                                                                               |                                                                                                           
ATATCACCGTACAGCTGCAGGGCGACGGTCCGATGAATCTGGCGGTTATTAACGGTAACAATAACCAGCAGATGCGCGGTGTGGCGCGCGTGCAGGGCGAAATTCCAGAAAATGCCGACCTGAAAACGCTGGTCGGCAATGGTTACGTGGTGATCACCATTACCCCGAGCGAAGGCGAACGCTATCAGGGCGTAGTTGGTCTGGAAGGTGATACCCTGGCGGCCTGCCTGGAAGA  >  NC_000913/3529964‑3530198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: