Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 4,283,098 | G→T | intergenic (‑23/+155) | yjcF ← / ← actP | pentapeptide repeats protein/acetate transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,283,098 | 0 | G | T | 95.2% | 66.6 / ‑2.2 | 21 | intergenic (‑23/+155) | yjcF/actP | pentapeptide repeats protein/acetate transporter |
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (10/10); total (10/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01 |
TTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAG > NC_000913/4282963‑4283211 | ttGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACaataaa > 1:277135/1‑139 (MQ=255) ttATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCa < 2:277135/139‑1 (MQ=255) aTCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAGGAAAAGGCata > 2:379090/1‑139 (MQ=255) cTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCa < 1:146750/139‑1 (MQ=255) cTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCa < 1:339064/139‑1 (MQ=255) gtgACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCaaaa > 2:440607/1‑139 (MQ=255) agTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATtct > 1:210992/1‑102 (MQ=255) agTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATtct < 2:210992/102‑1 (MQ=255) agTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTc < 1:512274/139‑1 (MQ=255) tCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTa < 1:466217/139‑1 (MQ=255) aTCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGt > 1:165302/1‑139 (MQ=255) gCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAataaagaa < 2:575588/71‑1 (MQ=255) gCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAataaagaa > 1:575588/1‑71 (MQ=255) ggAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATAATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAat > 1:47836/1‑139 (MQ=255) aaaCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAAtatta < 1:366760/139‑1 (MQ=255) aCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa < 1:361191/74‑1 (MQ=255) aCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCGCTGCa > 2:361191/1‑74 (MQ=255) tAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTg < 1:379090/139‑1 (MQ=255) ccATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGc > 1:470134/1‑139 (MQ=255) cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa > 1:419514/1‑49 (MQ=255) cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa < 2:419514/49‑1 (MQ=255) cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGtt > 1:58834/1‑96 (MQ=255) cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGtt < 2:58834/96‑1 (MQ=255) cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGagag > 1:372915/1‑139 (MQ=255) | TTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAG > NC_000913/4282963‑4283211 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |