Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,283,098 G→T intergenic (‑23/+155) yjcF ← / ← actP pentapeptide repeats protein/acetate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,283,0980GT95.2% 66.6 / ‑2.2 21intergenic (‑23/+155)yjcF/actPpentapeptide repeats protein/acetate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (10/10);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

TTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAG  >  NC_000913/4282963‑4283211
                                                                                                                                       |                                                                                                                 
ttGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACaataaa                                                                                                                >  1:277135/1‑139 (MQ=255)
         ttATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCa                                                                                                       <  2:277135/139‑1 (MQ=255)
           aTCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAGGAAAAGGCata                                                                                                     >  2:379090/1‑139 (MQ=255)
                          cTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCa                                                                                      <  1:146750/139‑1 (MQ=255)
                          cTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCa                                                                                      <  1:339064/139‑1 (MQ=255)
                                             gtgACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCaaaa                                                                   >  2:440607/1‑139 (MQ=255)
                                                    agTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATtct                                                                                                 >  1:210992/1‑102 (MQ=255)
                                                    agTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATtct                                                                                                 <  2:210992/102‑1 (MQ=255)
                                                    agTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTc                                                            <  1:512274/139‑1 (MQ=255)
                                                            tCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTa                                                    <  1:466217/139‑1 (MQ=255)
                                                                  aTCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGt                                              >  1:165302/1‑139 (MQ=255)
                                                                       gCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAataaagaa                                                                                                             <  2:575588/71‑1 (MQ=255)
                                                                       gCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAataaagaa                                                                                                             >  1:575588/1‑71 (MQ=255)
                                                                               ggAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATAATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAat                                 >  1:47836/1‑139 (MQ=255)
                                                                                   aaaCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAAtatta                             <  1:366760/139‑1 (MQ=255)
                                                                                     aCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa                                                                                            <  1:361191/74‑1 (MQ=255)
                                                                                     aCATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCGCTGCa                                                                                            >  2:361191/1‑74 (MQ=255)
                                                                                               tAAACCATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTg                 <  1:379090/139‑1 (MQ=255)
                                                                                                   ccATTATAGCGCATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGc             >  1:470134/1‑139 (MQ=255)
                                                                                                              cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa                                                                                            >  1:419514/1‑49 (MQ=255)
                                                                                                              cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCa                                                                                            <  2:419514/49‑1 (MQ=255)
                                                                                                              cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGtt                                             >  1:58834/1‑96 (MQ=255)
                                                                                                              cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGtt                                             <  2:58834/96‑1 (MQ=255)
                                                                                                              cATAAAATATCCTTATAATTAACAATAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGagag  >  1:372915/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                 
TTGCTGTAATTATCTGATTTTGTTTTCTTTGTATGTGATGGACTTGTGACAGAGTGGTTATCGTTAATCAGGCAAAGAGGGAAAAACATATTATTTAAACCATTATAGCGCATAAAATATCCTTATAATTAACAAGAAAGAAAAGGCATATTCTCTGCATTATCATTTTCTACGACTGTCAAAAATCGCTCATTTTTTAATGAGTTTATTTGTTTAATATTATGGGAAAAGGTGATGCATTTGGGAGAG  >  NC_000913/4282963‑4283211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: