Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,538,491 C→T G92D (GGC→GAC)  nanM ← N‑acetylneuraminic acid mutarotase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,538,4910CT97.6% 133.5 / NA 41G92D (GGC→GAC) nanMN‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (23/17);  total (24/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

TTGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGCCGTACCTGCG  >  NC_000913/4538356‑4538625
                                                                                                                                       |                                                                                                                                      
ttGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATgtcgc                                                                                                                                     <  2:555492/139‑1 (MQ=255)
  gtgtACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                                   <  1:138855/139‑1 (MQ=255)
     tACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAac                                                                                                                                >  1:542393/1‑139 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAg                                                                                                                        >  1:531961/1‑139 (MQ=255)
             tCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAg                                                                                                                        >  1:468899/1‑139 (MQ=255)
                         ccATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                            >  2:159911/1‑139 (MQ=255)
                                     gcgcGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGt                                                                                                <  2:468899/139‑1 (MQ=255)
                                          tGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                                   <  1:284428/99‑1 (MQ=255)
                                          tGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCa                                                                                                                                   >  2:284428/1‑99 (MQ=255)
                                                     tttAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGt                                                                                <  1:169464/139‑1 (MQ=255)
                                                             aaCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                            <  2:559230/103‑1 (MQ=255)
                                                             aaCTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAAt                                                                                                            >  1:559230/1‑103 (MQ=255)
                                                                     gTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGa                                                                      >  2:461477/1‑133 (MQ=255)
                                                                     gTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGa                                                                      <  1:461477/133‑1 (MQ=255)
                                                                               tGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                                 <  2:427328/64‑1 (MQ=255)
                                                                               tGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCaaa                                                                                                                                 >  1:427328/1‑64 (MQ=255)
                                                                                gTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATAc                                                                                                                          >  1:583344/1‑70 (MQ=255)
                                                                                gTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATAc                                                                                                                          <  2:583344/70‑1 (MQ=255)
                                                                                      gtgtACGTCATTAAATACCTGAGTCAAGCCCTCGCTATTTTTGCCAATGTCGCCAAACACATATAGATTGCCATCAATAAATGCAGATGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCAtttt                                               >  2:369987/1‑139 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATc                                          >  1:513922/1‑139 (MQ=255)
                                                                                           cGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATc                                          >  2:517388/1‑139 (MQ=255)
                                                                                                 tAAATACCTGAGTCAAGCCCTCGCTGCTTTTGCCAATCTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGc                                    <  1:369987/139‑1 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCag                                                                                                    <  1:502572/68‑1 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCag                                                                                                    >  2:502572/1‑68 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                             >  2:577396/1‑139 (MQ=255)
                                                                                                        cTGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTa                             >  1:348961/1‑139 (MQ=255)
                                                                                                         tGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                            <  2:531961/139‑1 (MQ=255)
                                                                                                         tGAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTAt                            <  1:28151/139‑1 (MQ=255)
                                                                                                          gAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACg                                                          <  1:110830/108‑1 (MQ=255)
                                                                                                          gAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACg                                                          >  2:110830/1‑108 (MQ=255)
                                                                                                          gAGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATc                           >  2:315112/1‑139 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTgg                                     <  2:30523/128‑1 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTgg                                     >  1:30523/1‑128 (MQ=255)
                                                                                                           aGTCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATcc                          >  2:180011/1‑139 (MQ=255)
                                                                                                             tCAAGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAg                        >  1:452722/1‑139 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATATCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCt                      >  1:531054/1‑139 (MQ=255)
                                                                                                               aaGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCt                      >  1:259350/1‑139 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTgg                                                                                 <  2:221514/73‑1 (MQ=255)
                                                                                                                      cGCTGTTTTTGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTgg                                                                                 >  1:221514/1‑73 (MQ=255)
                                                                                                                            ttttGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGcc                                                                           <  2:292211/73‑1 (MQ=255)
                                                                                                                            ttttGCCAATGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGcc                                                                           >  1:292211/1‑73 (MQ=255)
                                                                                                                                   aaTGTCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGCCGTACCTgcg  <  2:259350/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                      
TTGTGTACAAAAGTCACATGGCCCGCCATGCCCATCGGCGCGTGCGACATCAATTTAACCCAACTATTGGTTTTGGGGTTGTATTTGTGTACGTCATTAAATACCTGAGTCAAGCCCTCGCTGTTTTTGCCAATGCCGCCAAACACATACAGATTGCCATCAATAAATGCAGAGGTTGCTTGATCTCTTGGTCCGCCAGGGAATGCAGCTAACGCTGTCCATTTTTTATCTTTGGCCTGTGTATCCAGCTTGTACCATGCCGTACCTGCG  >  NC_000913/4538356‑4538625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑