Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,586,393	A→G	T123T (ACT→ACC)	hsdR ←	endonuclease R Type I restriction enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,586,393	0	A	G	100.0%	54.2 / NA	15	T123T (ACT→ACC)	hsdR	endonuclease R Type I restriction enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/9);  total (6/9)

GCCTGAGTCTGCGCTTTTTCTCGCACCTGCTGTTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGG  >  NC_000913/4586265‑4586501                                                                                                                                 |                                                                                                             gCCTGAGTCTGCGCTTTTTCTCGCACCTGCTGTTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGAc                                                                                                    <  2:136282/139‑1 (MQ=255)            cgcTTTTTCTCGCACCTGCTGTTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAg                                                                                         >  1:492819/1‑139 (MQ=255)                                                         cACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTg                                            <  1:203338/139‑1 (MQ=255)                                                                       agagGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTgaga                                         <  1:118952/128‑1 (MQ=255)                                                                       agagGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTgaga                                         >  2:118952/1‑128 (MQ=255)                                                                                   cacGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGc                  <  1:355661/139‑1 (MQ=255)                                                                                     cGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGtt                                             <  1:7269/110‑1 (MQ=255)                                                                                     cGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGtt                                             >  2:7269/1‑110 (MQ=255)                                                                                     cGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCt                <  2:492819/139‑1 (MQ=255)                                                                                                 acaAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAgg                                                              <  1:148459/81‑1 (MQ=255)                                                                                                 acaAACACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAgg                                                              >  2:148459/1‑81 (MQ=255)                                                                                                    aaCACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATgcgg  >  1:458235/1‑138 (MQ=255)                                                                                                    aaCACCGGCACCGGGAAGTCATAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATgcgg  <  2:458235/138‑1 (MQ=255)                                                                                                                        aTAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCAt                                                 >  1:466186/1‑71 (MQ=255)                                                                                                                        aTAATCTTTGGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCAt                                                 <  2:466186/71‑1 (MQ=255)                                                                                                                                 |                                                                                                             GCCTGAGTCTGCGCTTTTTCTCGCACCTGCTGTTCAAGCTGTTGTTTTAGCGTCAGCACTTCCTGGTGATAGAGGTTTTCACCACGTTCCGGCAACACAAACACCGGCACCGGGAAGTCATAATCTTTAGTGACCAGACGGTAGTACCAGACAGCCAGGCGGAACCCGAGTCGCAGGCACATCTGGGCATCGTTGAGATCGTTATGATATTCGTGCACCGCCTGGTTACCAATGCGG  >  NC_000913/4586265‑4586501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑