Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	749,989	A→G	G727G (GGT→GGC)	ybgQ ←	putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	749,989	0	A	G	100.0%	99.7 / NA	28	G727G (GGT→GGC)	ybgQ	putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/15);  total (13/15)

TTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAA  >  NC_000913/749854‑750098                                                                                                                                        |                                                                                                              ttcctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCACACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCt                                                                                                            <  1:62191/139‑1 (MQ=255)   cctCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTAt                                                                                                          >  1:448729/1‑139 (MQ=255)                     cGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGt                                                                                        >  2:396067/1‑139 (MQ=255)                     cGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGt                                                                                        <  2:150872/139‑1 (MQ=255)                           tGCCGCTGTCATCCTGGCGGATATGCGCACCGAGGGCAGGGAGCTGACCGCTGGTGTTGCGTATGGTGACGTGAACGTGTTTACCGGAACGGAAACCCAGTGAGTTGTAGCCTATCGTGTCATCAATTCATGTTTCCtt                                                                                  >  1:127670/1‑139 (MQ=255)                           tGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAAt                                                                                              >  2:498656/1‑127 (MQ=255)                           tGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAAt                                                                                              <  1:498656/127‑1 (MQ=255)                            gCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTtg                                                                                 <  2:59059/139‑1 (MQ=255)                                  gTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGATCGGGAAGCCAGAGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACg                                                                           <  1:396067/139‑1 (MQ=255)                                           gCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCa                                                                  >  2:456433/1‑139 (MQ=255)                                             ggATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGttt                                                                                      >  2:349681/1‑117 (MQ=255)                                             ggATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGttt                                                                                      <  1:349681/117‑1 (MQ=255)                                                           aggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCtt                                                                                                  <  1:305217/91‑1 (MQ=255)                                                           aggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCtt                                                                                                  >  2:305217/1‑91 (MQ=255)                                                            ggggagggAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCggg                                                 <  2:124230/139‑1 (MQ=255)                                                             gggagggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTa                                                           <  1:239967/128‑1 (MQ=255)                                                             gggagggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTa                                                           >  2:239967/1‑128 (MQ=255)                                                                agggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAg                                             >  2:169040/1‑139 (MQ=255)                                                                 gggaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGt                                            <  2:448729/139‑1 (MQ=255)                                                                   gaACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGtca                                          <  2:256643/139‑1 (MQ=255)                                                                      cTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGt                                                >  2:331651/1‑130 (MQ=255)                                                                      cTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGt                                                <  1:331651/130‑1 (MQ=255)                                                                                                    aaCGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTaa         >  1:19052/1‑139 (MQ=255)                                                                                                           ttACCGGAACGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGaaa  <  1:169040/139‑1 (MQ=255)                                                                                                                  aaCGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCa                          >  2:26306/1‑108 (MQ=255)                                                                                                                  aaCGGGAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCa                          <  1:26306/108‑1 (MQ=255)                                                                                                                      ggAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCgg                                                  <  2:121960/80‑1 (MQ=255)                                                                                                                      ggAAGCCAGTGATTTGTAGCCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCgg                                                  >  1:121960/1‑80 (MQ=255)                                                                                                                                        |                                                                                                              TTCCTCGCCAACCATACCCACGCTAATGCCGCTGTCATCCTGGCGGATATCCGCACCGAGGGGAGGGAACTGACCGCTGGCGTTGCGTATGATGACGTTAACGTCTTTACCGGAACGGGAAGCCAGTGATTTGTAACCTATCGCGCCTTCAATCCATGTTTCCTTGATGACGTTTTCTGCAACTGTTACGCCGTCGGGTAAGTCATTCATGTTCACCGCCACGGTGGAAGGCTGGTAACTGGAAA  >  NC_000913/749854‑750098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑