| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,350,675 | Δ1 bp | noncoding (99/121 nt) | arcZ → | sRNA positive antisense regulator of rpoS, binds Hfq |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,350,672 | 0 | C | . | 96.2% | 105.4 / ‑1.6 | 26 | noncoding (96/121 nt) | arcZ | sRNA positive antisense regulator of rpoS, binds Hfq |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (14/11); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
TGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCA > NC_000913/3350534‑3350803 | tGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCAc < 2:198634/139‑1 (MQ=255) cAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCg < 2:451564/139‑1 (MQ=255) ttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGt < 1:189007/139‑1 (MQ=255) ttGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGt > 2:189007/1‑139 (MQ=255) gtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCAcc > 2:355889/1‑97 (MQ=255) gtgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCAcc < 1:355889/97‑1 (MQ=255) tgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg > 1:222720/1‑139 (MQ=255) tgcgGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACg > 2:35751/1‑139 (MQ=255) cTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGCATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCa < 1:405265/139‑1 (MQ=255) tGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAg < 1:404296/139‑1 (MQ=255) gTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTa < 2:610856/139‑1 (MQ=255) cTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTCGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcgtc > 2:493616/1‑139 (MQ=255) aCTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCActct > 2:653754/1‑139 (MQ=255) taatgaTTTACGGCGTAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCAt < 1:240789/139‑1 (MQ=255) taataaTTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCAt < 1:653754/139‑1 (MQ=255) tACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCaa > 2:255651/1‑139 (MQ=255) tACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCaa > 1:120680/1‑139 (MQ=255) ggCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCAc > 1:60143/1‑44 (MQ=255) ggCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCAc < 2:60143/44‑1 (MQ=255) cgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc < 2:662588/83‑1 (MQ=255) cgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccaccc > 1:662588/1‑83 (MQ=255) cgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccac < 2:488283/81‑1 (MQ=255) cgcAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGccac > 1:488283/1‑81 (MQ=255) ccAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcg < 2:35750/98‑1 (MQ=255) ccAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTAcg > 1:35750/1‑98 (MQ=255) tgtTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCAt < 1:641790/41‑1 (MQ=255) tgtTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCAt > 2:641790/1‑41 (MQ=255) tgtTGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCa > 1:663241/1‑139 (MQ=255) tGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACtcttct < 2:208284/98‑1 (MQ=255) tGGCGCAGTATTCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACtcttct > 1:208284/1‑98 (MQ=255) ttCGCGCA‑CCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCa > 1:264507/1‑139 (MQ=255) | TGATTCATGTAACAAATCATTTAAGTTTTGCTATCTTAACTGCGTGCGGCCTGAAAAACAGTGCTGTGCCCTTGTAACTCATCATAATAATTTACGGCGCAGCCAAGATTTCCCTGGTGTTGGCGCAGTATTCGCGCACCCCGGTCTAGCCGGGGTCATTTTTTAGTGGCTTTTGCCACCCACGCTTTCAGCACTTCTACGTCGTGACGCCACTCTTCTTTCATCTCTTCAATCCATTCACCGACGTTATCTTCCCAGGCCGGAAGGTCA > NC_000913/3350534‑3350803 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |