| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 367,852 | (GC)6→5 | coding (568‑569/834 nt) | mhpR ← | mhp operon transcriptional activator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 367,842 | 0 | G | . | 87.5% | 47.7 / 3.8 | 16 | coding (579/834 nt) | mhpR | mhp operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (7/7); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.26e-01 | |||||||||||
| * | NC_000913 | 367,843 | 0 | C | . | 87.5% | 48.1 / 4.0 | 16 | coding (578/834 nt) | mhpR | mhp operon transcriptional activator |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base . (7/7); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGC > NC_000913/367708‑367965 || aTGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcg < 2:145751/139‑1 (MQ=255) gCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCt > 1:503342/1‑139 (MQ=255) gCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCt > 2:51473/1‑139 (MQ=255) ccATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCtt > 2:106473/1‑139 (MQ=255) gATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTaatg > 1:50007/1‑137 (MQ=255) aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgc < 1:544896/113‑1 (MQ=255) aTTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTgcgcgcgc > 2:544896/1‑113 (MQ=255) cACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTg < 1:312778/139‑1 (MQ=255) aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg < 1:704673/131‑1 (MQ=255) aCCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATATTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTg > 2:704673/1‑131 (MQ=255) gcAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCGCTTT‑‑GCGCGCGCGCCAGAAGGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGc < 2:68949/139‑1 (MQ=255) cGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGa < 2:246253/139‑1 (MQ=255) tcctccTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGTCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg < 2:373641/139‑1 (MQ=255) tcctccTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAATGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTg < 2:50007/139‑1 (MQ=255) cctGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGt > 2:220635/1‑139 (MQ=255) aGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTc > 1:609777/1‑139 (MQ=255) tCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGgccagc < 2:609777/139‑1 (MQ=255) tAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAgg < 2:31440/128‑1 (MQ=255) tAACCCTCTTT‑‑GCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAgg > 1:31440/1‑128 (MQ=255) || ATGGTCATTGCGCTCGCCATATACACCAGATTCAGACAGCCAATCACCCGTTGTTCACTGCGCAGCGGTACGGCGATAGAGGCGATCTTCTCCTCCTGATCCCAGCCGCGGTAGTTCTGTCCGTAACCCTCTTTGCGCGCGCGCGCCAGAATGGCTTCCAGCTTTAACGGTTCCCGTGCCAGTTGATAGTCATCACCGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGTTCCGGGCAAAAGGCCAGC > NC_000913/367708‑367965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |