Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,002,224 (G)7→8 coding (418/1071 nt) elfG → putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,002,2171.G80.0% 25.4 / 2.0 10coding (411/1071 nt)elfGputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (3/5);  total (3/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTC  >  NC_000913/1002086‑1002333
                                                                                                                                    |                                                                                                                     
ttCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑ggggggg                                                                                                               >  1:383959/1‑139 (MQ=255)
      gcgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATAcc                                                                                                          <  2:383959/139‑1 (MQ=255)
       cgGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATAccc                                                                                                         <  1:702801/139‑1 (MQ=255)
                               gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTg                                                                                            >  1:92727/1‑128 (MQ=255)
                               gCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTg                                                                                            <  2:92727/128‑1 (MQ=255)
                                                           tCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAg                                                     <  2:347922/139‑1 (MQ=255)
                                                                                                            gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTc                                                                              <  1:136028/65‑1 (MQ=255)
                                                                                                            gTTCCTTTCGAAAACGTGGGCACAGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTc                                                                              >  2:136028/1‑65 (MQ=255)
                                                                                                             ttCCTTTCGAAAACGTGGGCACA‑‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTc  <  1:131017/139‑1 (MQ=255)
                                                                                                             ttCCTTTCGAAAACGTGGGCACA‑‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTc  <  2:360858/139‑1 (MQ=255)
                                                                                                              tCCTTTCGAAAACGTGGGCACAGGGGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCt   >  2:867/1‑139 (MQ=255)
                                                                                                                                    |                                                                                                                     
TTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGACAACTGGTACAAAATTAATGACTATCTTGCCGCCAAAGTTTTATTGCAGGTTAAAGGCAGTTCTCCTACTGCGGTTCCTTTCGAAAACGTGGGCACA‑‑GGGGGGGATACCCGATGGCATATTTGCGACCCTGGCGGTCAACGTTTAGGTGGGCAGGGGGCAAGCGGTAATAGCGGTAGCTTTTCCCTGAAAATATTGCAGCCGTTCGTTGGCTC  >  NC_000913/1002086‑1002333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: