| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,288,561 | (C)7→8 | pseudogene (354/2525 nt) | yejO ← | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,288,554 | 1 | . | C | 81.6% | 109.8 / 20.0 | 38 | pseudogene (361/2525 nt) | yejO | pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system |
| Reads supporting (aligned to +/- strand): ref base . (4/3); new base C (15/16); total (19/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
CCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAT > NC_000913/2288416‑2288683 | ccGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACg‑g > 1:196006/1‑139 (MQ=255) cGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCTCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑c < 2:223145/139‑1 (MQ=255) tttGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑c‑ccccccca > 1:231878/1‑137 (MQ=255) aaTACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCaa > 2:604824/1‑139 (MQ=255) ccATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTaa > 2:56542/1‑139 (MQ=255) gcgTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGcgcccgc > 1:140285/1‑139 (MQ=255) tttAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGt < 1:595027/139‑1 (MQ=255) aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTa > 2:228527/1‑139 (MQ=255) aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATc < 2:547867/132‑1 (MQ=255) aTAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATc > 1:547867/1‑132 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGAtt < 1:219935/106‑1 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGAtt > 2:219935/1‑106 (MQ=255) ccTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCt > 2:716589/1‑139 (MQ=255) tCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCAt < 2:140285/139‑1 (MQ=255) aTCTACCGCAAATACAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAc < 2:369961/139‑1 (MQ=255) tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGcgcc > 2:517401/1‑99 (MQ=255) tACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGcgcc < 1:517401/99‑1 (MQ=255) cGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCACCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAg > 1:579586/1‑139 (MQ=255) caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca < 1:716589/139‑1 (MQ=255) caaatgcaaatCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca < 2:484775/139‑1 (MQ=255) cccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGc < 1:229348/139‑1 (MQ=255) ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc > 1:78853/1‑104 (MQ=255) ccTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGc < 2:78853/104‑1 (MQ=255) tctTTTTCCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAATACG‑G‑CTCCCCCCATCTTTTTCAACTATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTa < 2:365016/139‑1 (MQ=255) tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt > 2:8593/1‑83 (MQ=255) tAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAAt < 1:8593/83‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa > 2:383733/1‑98 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCa < 1:383733/98‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca > 2:401703/1‑111 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca < 1:401703/111‑1 (MQ=255) cAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGc > 2:397228/1‑139 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc > 1:317319/1‑102 (MQ=255) agtagtCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAAcc < 2:317319/102‑1 (MQ=255) agtCCTCCCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCcgcg < 2:231878/139‑1 (MQ=255) tcctccCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa < 1:534730/117‑1 (MQ=255) tcctccCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGa > 2:534730/1‑117 (MQ=255) ctccCTTTTCGATAGCGACAAAGATG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc > 2:222206/1‑139 (MQ=255) ctccCTTTTCGATAGCGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCAc > 1:224646/1‑139 (MQ=255) ttCGATAGCGACAAAGATG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTg < 1:222206/139‑1 (MQ=255) cGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca > 1:492375/1‑88 (MQ=255) cGACAAAGACG‑GCC‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGca < 2:492375/88‑1 (MQ=255) cAAAGACGCC‑C‑CCCCCCATCTTTTGCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAt < 2:579586/139‑1 (MQ=255) | CCGAGACGGTTTGTTCCGAATACCTCCATGGCCCGCGTGGTTGTTTTAATAGCACCGCCTGCTTTCTGATCTACCGCAAATGCAAATCCCCCCTCTTTAACCTCCAGTAGTCCTCCCTTTTCGATAGCGACAAAGACG‑G‑C‑CCCCCCATCTTTTTCAACGATTAAGCGCCCGCCTTTAATCGTGGTATCATTCGCTGCGCCATAAACCTGTAGCACACCATTATTGAGCGTAGTCTGGTTTGCCGCGCCACCCATTTGAACAATTTGAT > NC_000913/2288416‑2288683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |