| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,763,869 | (T)7→8 | coding (1285/1617 nt) | yfjL ← | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,763,862 | 1 | . | T | 96.5% | 93.0 / ‑3.0 | 29 | coding (1292/1617 nt) | yfjL | CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base T (14/14); total (14/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.19e-01 | |||||||||||
ATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑A‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGC > NC_000913/2763735‑2763981 | aTTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑A‑ttttttttgcg < 1:265092/139‑5 (MQ=255) tAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTgcgcg < 1:608304/139‑1 (MQ=255) agagTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTgcgcgc > 1:179829/1‑139 (MQ=255) gCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGAt < 2:79054/139‑1 (MQ=255) cGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAAt > 1:238333/1‑139 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 1:392249/139‑1 (MQ=255) aaCGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTcc < 2:179829/139‑1 (MQ=255) gAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTa < 2:238333/139‑1 (MQ=255) cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGaa > 2:376641/1‑120 (MQ=255) cGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGaa < 1:376641/120‑1 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTgcgcg > 1:153130/1‑91 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTgcgcg < 2:153130/91‑1 (MQ=255) cATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTAtct > 1:113041/1‑139 (MQ=255) ccATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:113041/139‑1 (MQ=255) ccATAAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGGCT‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCa < 2:639102/139‑1 (MQ=255) tCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAatat > 1:674584/1‑137 (MQ=255) tCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAatat < 2:674584/137‑1 (MQ=255) tGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCa > 2:215837/1‑139 (MQ=255) aaGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGaa > 1:64551/1‑139 (MQ=255) aGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATg < 1:42193/55‑1 (MQ=255) aGCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATg > 2:42193/1‑55 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACaa < 2:278512/77‑1 (MQ=255) gCAGGTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACaa > 1:278512/1‑77 (MQ=255) gTAGTTATCGTCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAAt > 2:217480/1‑139 (MQ=255) tCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg > 1:530515/1‑92 (MQ=255) tCTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACgg < 2:530515/92‑1 (MQ=255) cTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGc < 2:343877/135‑1 (MQ=255) cTTTAATGTCGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGc > 1:343877/1‑135 (MQ=255) cGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGACAATGTTAATATCAGTCACCAACTCGCt > 1:89906/1‑110 (MQ=255) cGAGG‑ATTTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGACAATGTTAATATCAGTCACCAACTCGCt < 2:89906/110‑1 (MQ=255) | ATTAGAGTCGTAGCCCACGAACAGAACAAACGTGAAACGATCGAGGTGAGCATCGAAAGGTTGGCTGCCATCAAGAATTTCGTCAATGTCGTGGCGAAGCAGGTAGTTATCGTCTTTAATGTCGAGG‑A‑TTTTTTTGCGCGCGGTATCGATGCAATCCGAAATGTCCTCGTACAACTGATCCCGTATCTCCGGCAATCTGACGGCAATGTTAATATCAGTCACCAACTCGCTGAAGCCAATCCAAAGC > NC_000913/2763735‑2763981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |