Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,199,3430CT70.3% 42.4 / 29.4 37R100H (CGC→CAC) glnEfused deadenylyltransferase/adenylyltransferase for glutamine synthetase
Reads supporting (aligned to +/- strand):  ref base C (6/5);  new base T (14/12);  total (20/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.84e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCC  >  NC_000913/3199220‑3199474
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tCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGcgcc                                                                                                                      >  2:598437/1‑139 (MQ=255)
  gcgGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGcgccgc                                                                                                                    >  2:533347/1‑139 (MQ=255)
          aagCGTCATACAGCCCGTCACGCGCCGCAACAATCAGCGTCTCCTCCAGGTAGCTGATCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCTCTGGCGATGCGCCCCATAAAGCCACGCCCGAATac                                                                                                            >  2:44599/3‑138 (MQ=255)
                       ccAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCTAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCgcgc                                                                                               >  2:670523/1‑139 (MQ=255)
                        cAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCa                                                                                              >  2:547491/1‑139 (MQ=255)
                                    gcAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCtt                                                                                  >  1:162740/1‑139 (MQ=255)
                                        caaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtc                                                                              <  1:630405/139‑1 (MQ=255)
                                        caaTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtc                                                                              <  1:598437/139‑1 (MQ=255)
                                          aTCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCTAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcac                                                                            <  1:670523/139‑1 (MQ=255)
                                           tCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATg                                                                                                                          <  2:707218/92‑1 (MQ=255)
                                           tCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATg                                                                                                                          >  1:707218/1‑92 (MQ=255)
                                             aGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcactca                                                                         >  1:132395/1‑139 (MQ=255)
                                                   tcCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTAc                                                                   <  1:533347/139‑1 (MQ=255)
                                                          gATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAgcgc                                                            <  1:547491/139‑1 (MQ=255)
                                                           aTAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGcc                                                           <  1:674374/139‑1 (MQ=255)
                                                                            atatGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCg                                                                                                         >  1:627550/1‑76 (MQ=255)
                                                                            atatGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCg                                                                                                         <  2:627550/76‑1 (MQ=255)
                                                                             tatGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCcgcg                                         <  1:368048/139‑1 (MQ=255)
                                                                                    tctTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAAtgct                                  >  1:45740/1‑139 (MQ=255)
                                                                                      ttCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGaa                                                                                                               <  1:292795/60‑1 (MQ=255)
                                                                                      ttCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGaa                                                                                                               >  2:292795/1‑60 (MQ=255)
                                                                                             aCCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCtt                                                                                  <  1:266438/82‑1 (MQ=255)
                                                                                             aCCAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCtt                                                                                  >  2:266438/1‑82 (MQ=255)
                                                                                              ccAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGaa                                                                                                               >  1:148647/1‑52 (MQ=255)
                                                                                              ccAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGaa                                                                                                               <  2:148647/52‑1 (MQ=255)
                                                                                              ccAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcactc                                                                          >  2:212883/1‑89 (MQ=255)
                                                                                              ccAGTGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtcactc                                                                          <  1:212883/89‑1 (MQ=255)
                                                                                                aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtc                                                                              >  2:260253/1‑83 (MQ=255)
                                                                                                aGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGtc                                                                              <  1:260253/83‑1 (MQ=255)
                                                                                                  tGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTc                                                                                                   >  2:356929/1‑60 (MQ=255)
                                                                                                  tGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTc                                                                                                   <  1:356929/60‑1 (MQ=255)
                                                                                                  tGCCAGCGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTGCGTCACTCACATTAGAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGcc                    >  1:427422/1‑139 (MQ=255)
                                                                                                  tGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAgcg                                                             >  1:593756/1‑98 (MQ=255)
                                                                                                  tGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAgcg                                                             <  2:593756/98‑1 (MQ=255)
                                                                                                        cGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAgcgc                                                            <  2:514065/93‑1 (MQ=255)
                                                                                                        cGTTTGCGCCCAGGCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAgcgc                                                            >  1:514065/1‑93 (MQ=255)
                                                                                                                    ggCGATGTGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTcc  >  2:82880/1‑139 (MQ=255)
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TCGCGGCAGCAGGCGTCATACAGCCAGTCACGCGCCGCAACAATCAGCGTCTCCGCCAGATAGCTGAGCTGCTGCAATATGCTCTCTTCAGTAACCAGTGCCAGCGTTTGCGCCCAGGCGATGCGCACCATAATGCGCCGCCGGAATAGCCGCAGCTCGCGCATTAACCCGGCTTCGTCACTCACATTACAGAGCGCCTCCTGCAACCATGCCGCGTAATGCTGCCATTCGTCGGCCTGCGGCGGTTGGCTTTCC  >  NC_000913/3199220‑3199474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑