| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 30,615 | C→T | P322L (CCT→CTT) | carA → | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 30,615 | 0 | C | T | 79.2% | 39.8 / 9.6 | 24 | P322L (CCT→CTT) | carA | carbamoyl phosphate synthetase small subunit, glutamine amidotransferase |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (11/8); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.41e-01 | |||||||||||
GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGTTGTTC > NC_000913/30481‑30751 | ggcgAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCa < 2:472354/139‑1 (MQ=255) aaaaTGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAAt < 1:550955/139‑1 (MQ=255) aTTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTc > 1:247967/1‑139 (MQ=255) ttGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACACATAAATCCCTGTTCGa < 2:115594/139‑1 (MQ=255) caccacGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa < 1:166913/139‑1 (MQ=255) aaGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGa > 1:319949/1‑139 (MQ=255) aaaaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCAt > 2:105657/1‑139 (MQ=255) aaaCGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTc > 1:110350/1‑139 (MQ=255) cACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAc > 2:129373/1‑139 (MQ=255) ccccccAGAACAACGGTTTTGCGGTGGACTAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATTCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCAccc > 2:415932/4‑139 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa < 2:434538/78‑1 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCt > 1:368882/1‑139 (MQ=255) cGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTCTTCGa > 1:434538/1‑78 (MQ=255) cccAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGTATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGa > 1:251731/1‑139 (MQ=255) cAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa < 2:252300/79‑1 (MQ=255) cAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTa > 1:252300/1‑79 (MQ=255) aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc < 2:371552/139‑1 (MQ=255) aGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagc > 1:198023/1‑139 (MQ=255) aaCCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa > 1:585895/1‑71 (MQ=255) aaCCACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGa < 2:585895/71‑1 (MQ=255) cACGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAagccagcc < 2:247967/139‑1 (MQ=255) cGGTTTTGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCt > 1:502466/1‑139 (MQ=255) ttGCGGTGGACGAAGCAACATTACTTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTcc > 1:220172/1‑139 (MQ=255) aCCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGTTGTTc < 1:105657/139‑1 (MQ=255) ccTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACacgacg < 1:168093/124‑1 (MQ=255) ccTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACacgacg > 2:168093/1‑124 (MQ=255) | GGCGAGCGGTGCGAAGACTGTCAAAATGAAATTTGGTCACCACGGCGGCAACCATCCGGTTAAAGATGTGGAGAAAAACGTGGTAATGATCACCGCCCAGAACCACGGTTTTGCGGTGGACGAAGCAACATTACCTGCAAACCTGCGTGTCACGCATAAATCCCTGTTCGACGGTACGTTACAGGGCATTCATCGCACCGATAAACCGGCATTCAGCTTCCAGGGGCACCCTGAAGCCAGCCCTGGTCCACACGACGCCGCGCCGTTGTTC > NC_000913/30481‑30751 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |